Picture from Sun H et al. (2023) MicroPubl Biol "Comparing engineered nuclear-localized reporter cassettes."

Figure 1. Comparing engineered nuclear-localized reporter cassettes:Fig 1. (A) Schematic of reporter cassette comparison. (B) Comparison of the different reporter cassettes for neuropeptide nlp-51. Analysis for nlp-51 GFP fluorescence in the RIP and AIM neurons is shown on the left, with representative images on the right. (C) Comparison of the different reporter cassettes for neuropeptide flp-20. Analysis for flp-20 GFP fluorescence in the LUA, PVR, PVC, and PLM neurons is shown on the left, with representative images on the right. For B and C, *P<0.05, **P<0.01, ***P<0.001 posthoc t-test with Tukey multiple comparison correction. The black bars on the bottom right corner of microscope images represent 10 µm. (D) Summary of T2A::3xNLS::GFP vs. SL2::GFP::H2B comparisons across 6 different neuropeptide genes. Expression is determined using the reporter alleles in combination with NeuroPAL (Yemini et al., 2021), and the expression pattern of the SL2::GFP::H2B cassette more closely matches scRNA-seq data (Taylor et al., 2021).

Picture from Cook, Steven J. et al. (2021) MicroPubl Biol

Figure 1. AVH and AVJ neurons and hlh-34 expression: A: AVH and AVJ display a nearly identical position and axon trajectory in the lateral ganglion of C. elegans. Images used with permission from Wormatlas. B: Whole-animal lateral and dorsoventral overviews of hlh-34prom::gfp (otIs768 transgene) expressing young larvae. Animals imaged in a NeuroPAL (otIs669) background with only GFP emission shown for simplicity. C: Expression pattern of otIs768 and sIs14542 transgenic reporters in a NeuroPAL (otIs669) background. GFP signal is only observed in the AVH neuron. D: Embryonic expression pattern of otIs768. At ~360 minutes the GFP signal is observed in the AVH neuron and its dying sibling cell. All animals were imaged in a NeuroPAL (otIs669) background with only GFP emission shown for simplicity. E: Lineages of AVH/J in red. CEPsoDL/R shown in orange. Dying siblings of AVH shown as 'x'. F: hlh-34 locus and promoter fragment used in the sIs14542 transgene. Coordinates are V:13709947..13712813 in WS140 and V:13707915..13707382 in WS280. The size of the promoter used in the leEx1692 strain, kindly provided by Ian Hope, could not be unambiguously determined. The strain used in the first publication on hlh-34 (Cunningham et al. 2012) used 2.5kb upstream of hlh-34. G: Heatmap of scRNA expression (Taylor et al. 2021), showing hlh-34 expression and other markers of AVH and AVJ identity.