Picture from Siete C et al. (2024) MicroPubl Biol "Immobilization of C. elegans with different concentrations of an ...."

Figure 1. Comparison of axonal transport of TIR-1:GFP in AWC axons of worms immobilized with different concentrations of tetramisole and polystyrene microbeads:(A) Representative kymographs of moving TIR-1::GFP in AWC axons of worms immobilized with 0.5-7.5 mM tetramisole (Tet) or microbeads. The line within two blue arrowheads represents a retrograde trafficking event toward the proximal axon (P) or the ­­­­­AWC cell body; the line between two red arrowheads represents an anterograde trafficking event toward the distal (D) axon. (B) The percentage of anterograde and retrograde trafficking events. The average number of trafficking events per animal imaged is 19 (0.5 mM Tet), 24 (1 mM Tet), 21 (2 mM Tet), 19 (7.5 mM Tet), and 13 (microbeads). Z-test was used to determine p-values. Error bars, SE of proportion. ns, not significant. (C) The average velocity of anterograde and retrograde trafficking events. Red and blue lines indicate the comparison of anterograde and retrograde velocities, respectively. Student's t-test was used to determine p-values. Error bars, SEM. ns, not significant. The significance level 0.01 was adjusted to 0.0025 (0.01/4) using the Bonferroni method (Chen, Feng, & Yi, 2017) in (B) and (C).