Figure 3. Expression of GFP-tagged FLR-4 protein. (A-C) Nomarski images and (D-F) epifluorescence images of the corresponding animals of N2 carrying pMTG7-11 (GFP-tagged
flr-4). (A and D) Early L1 larva. (B and E) Comma stage embryo. (C and F) Three-fold stage embryo. (G) Ventral view of an adult head region, showing that the fluorescence of FLR-4::GFP is detected in AUA neurons as well as the muscles of the pharyngeal isthmus. The DiQ fluorescent marker, which penetrates into some neurons (not including AUA), is shown in red. Scale bar, 50 um.