Picture from Takashima Y et al. (2007) Gene "A novel non-coding DNA family in Caenorhabditis elegans."

Fig. 7. The expression patterns of C46H11.6::gfp and pat-10::lacZ reporter genes associated with or without CE1(bs258), and lacZ enhancer assay of CE1(bs258). (a) Schematic structure and summary of expression patterns of C46H11.6::gfp are shown. (b-g) C46H11.6::gfp reporter plasmids associated with CE1(bs258) (pC46H11BS1888 and pC46H11BB1439) were expressed in body-wall (d) and vulval muscles (e) and anal depressor muscle (f), and weakly expressed in intestinal cells, whereas C46H11.6::gfp reporter expressions without CE1(bs258) (pC46H11BB1164 and pC46H11BE1085) were hardly detectable in these somatic tissues (g). (h) Schematic structure and summary of lacZ enhancer assay of CE1(bs258) are shown. (i and j) The assay vector pPD95HHCE1bs258 was expressed and lacZ-stained in intestinal cells, whereas the control vector pPD95.18 was not expressed in any of tissues on its own. (k) Schematic structure and summary of expression patterns of pat-10::lacZ are shown (Terami et al., 1999). All reporter constructs of pat-10::lacZ associated with CE1(bs258) (pTNCZ7600), with the partial fragment of CE1(bs258) (pTNCZ1248), and without CE1(bs258) (pTNCZ647) were expressed in body-wall and vulval muscles and anal depressor muscle. Plus (+) and minus (-) indicate the intensity of reporter gene expressions, respectively. The scale bars represent 50 um.

Picture from Takashima Y et al. (2007) Gene "A novel non-coding DNA family in Caenorhabditis elegans."

(b-g) C46H11.6::gfp reporter plasmids associated with CE1(bs258) (pC46H11BS1888 and pC46H11BB1439) were expressed in body-wall (d) and vulval muscles (e) and anal depressor muscle (f), and weakly expressed in intestinal cells, whereas C46H11.6::gfp reporter expressions without CE1(bs258) (pC46H11BB1164 and pC46H11BE1085) were hardly detectable in these somatic tissues (g). The scale bars represent 50 um.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.

Picture from Millonigg S et al. (2014) PLoS Genet "GLD-4-mediated translational activation regulates the size of the ...."

(A) GLD-4 expression is equal across the distal germ line. GLD-2 intensities increase from low-to-high in a distal-to-proximal manner. Extruded gonads of indicated genotype stained with DAPI, a-GLD-2, a-GLD-4, and a-GLH-2 as a positive tissue penetration control (not shown). Asterisk, distal tip; arrowhead, mitosis-to-meiosis boundary.

Picture from Xu XZS et al. (2003) Cell "A C. elegans sperm TRP protein required for sperm-egg interactions during ...."

Figure 3. The TRP-3 Protein Is Highly Enriched in Sperm. (A-H) An affinity-purified antibody against TRP-3 specifically stains sperm in wild-type hermaphrodites. Scale bar = 10 μm.(A) An immunofluorescence image of anti-TRP3 staining of a wild-type hermaphrodite.(B) The same animal in (A) stained with DAPI.(C) A superimposed image of (A) and (B).(D) A DIC image of the same animal in (A-C).(E-H) The TRP-3 antibody is specific.(E) The TRP-3 antibody does not detect a signal in trp-3(sy693) mutants.(F) A DAPI image of the same animal shown in (E).(G) A superimposed image of (E) and (F).(H) A DIC image of the same animal shown in (E-G).

Picture from Chitturi J et al. (2018) PLoS Genet "The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate ...."

A) A confocal image ofanimals expressing a functional UBR-1::GFP transgene from its endogenous promoter(green), and the Pnmr-1-RFP reporter (red).