Picture from Nguyen TQ et al. (2000) J Cell Sci "The C. elegans septin genes, unc-59 and unc-61, are required for normal ...."

(A-F) illustrate that UNC-59 co-localizes with the actomyosin contractile ring. (A-C) A single embryo with the cleavage furrow about three-quarters ingressed; (D-F) a single embryo with the cleavage furrow fully ingressed. (A and D) Actin; (B and E) UNC-59;(C and F) merged images. Bar, 10 um.

Picture from Nguyen TQ et al. (2000) J Cell Sci "The C. elegans septin genes, unc-59 and unc-61, are required for normal ...."

Fig. 5. UNC-61 localizes to the border of the rachis of the syncytial germ cell in the ovary. Wild-type young adult animals were double labeled with UNC-61 antibody (green) and Tro-Pro-3 staining the germ cell nuclei (red). (A) shows a merged image of a whole- mount animal showing the ovary (bottom left). Similar patterns of staining were seen in the syncytial germ cell of the testis (not shown). (B and C) Ventral and lateral views of the transient UNC-61 staining that was observed at the onset of male tail morphogenesis in the early L4. Bar, 10 um.

Picture from Nguyen TQ et al. (2000) J Cell Sci "The C. elegans septin genes, unc-59 and unc-61, are required for normal ...."

(G-L) Embryos were double- labeled with non-muscle myosin II (NMY II)(red) and UNC-61 antibodies (green). The rows show the same embryo taken at the same focal plane. Similar to NMY-II (G), UNC-61 localizes to the leading edge of the cleavage furrow (H). The image (I) is a merged image of the NMY-II and UNC-61. At the terminal phase of cell division, both NMY-II (J) and UNC-61 (K) co-localizes to the midbody region. The image (L) is a merged image of J and K. Bar, 10 um.

Picture from Nguyen TQ et al. (2000) J Cell Sci "The C. elegans septin genes, unc-59 and unc-61, are required for normal ...."

Fig. 6. The distribution of UNC-59 and UNC-61 in the cleavage furrow and the midbody region.(A-F) illustrate that UNC-59 co-localizes with the actomyosin contractile ring. (A-C) A single embryo with the cleavage furrow about three-quarters ingressed; (D-F) a single embryo with the cleavage furrow fully ingressed. (A and D) Actin; (B and E) UNC-59;(C and F) merged images. (G-L) Embryos were double- labeled with non-muscle myosin II (NMY II)(red) and UNC-61 antibodies (green). The rows show the same embryo taken at the same focal plane. Similar to NMY-II (G), UNC-61 localizes to the leading edge of the cleavage furrow (H). The image (I) is a merged image of the NMY-II and UNC-61. At the terminal phase of cell division, both NMY-II (J) and UNC-61 (K) co-localizes to the midbody region. The image (L) is a merged image of J and K. Bar, 10 um.