Picture from Suzuki M et al. (2004) Biol Chem "Metalloproteases with EGF, CUB, and thrombospondin-1 domains function in ...."

Figure 2. Expression pattern of nas-37p::gfp. DNA fragment extending from y3679 to q5 relative to the initiation ATG codon of the nas-37 gene was amplified from the genomic DNA extracted from C. elegans N2 animals with the primer 5'-GCG CAA GCT TTC ACC CAA AAA TTA TCA AGG - 3' and 5'-GGC CGT CGA CTT CAT TCT GCA AAA TAG AAC-3'. The fragment was digested with HindIII and SalI, and ligated into the promoterless vector pPD95.67, allowing in-frame fusion of the nas-37 sequence and the GFP reporter gene. Transgenic strains were generated by microinjecting the reporter plasmid (50 ug/ul) together with the marker plasmid pRF-4 rol-6 (su1006) as described previously by Kramer et al. (1990) (50 ug/ ul) into the syncytial gonad of wild-type nematodes (Mello et al., 1991). Panels (A) and (B) show L3 larvae. The large and small arrows in the panel (A) indicate the hypodermal and rectal grand cells, respectively. Panel (C) shows a young adult hermaphrodite. The arrow indicates the position of vulva. Panel (D) shows an adult hermaphrodite. The left is a confocal image and the right is the merge with its bright field image. The arrow indicates the vulva.

Picture from Suzuki Y et al. (2002) Genetics "A cuticle collagen encoded by the lon-3 gene may be a target of TGF-beta signaling ...."

Figure 4.-Expression patterns of lon-3::gfp reporter transgenes. Integrated lines carrying lon-3::gfp reporter genes were generated as described in materials and methods. (A) L4 hermaphrodite carrying a transcriptional reporter gene with an SV40 nuclear localization signal (pYSL3G1) as an integrated array (kuIs56). GFP is localized to the nuclei of hypodermal cells; many are out of focus. Asterisks indicate nuclei of lateral hypodermal cells (seam cells). (B) Head region of an adult hermaphrodite carrying a translational fusion construct (pYSL3G3) as an integrated array (kuIs55). Note the GFP expression outlining the animal. (C-G) Comparison of GFP expression levels from kuIs55 in dbl-1(+), dbl-1(lf ), and dbl-1 overexpression (kuEx136) genetic backgrounds. These animals are at precisely the same stage based on vulval morphology. C and D show dbl-1(+) and dbl-1(wk70) animals. In D, the contrast was adjusted to visualize the low level of GFP expression in the dbl-1(+) animal. E-G are three of the photographs used to generate Table 4, showing animals with close to the average rating of GFP expression among the dbl- 1(+), dbl-1(nk3), and kuEx136 groups, respectively. The same exposure setting was used for all the images in E-G with no contrast adjustment. Light spots in E-G are caused by autofluorescence of gut granules and are unrelated to lon-3 expression.

Picture from Suzuki Y et al. (2006) Genes Dev "Genetic redundancy masks diverse functions of the tumor suppressor gene PTEN ...."

Figure S1. daf-18 mRNA is present in the germ line. Animals of the indicated genotypes were examined using in situ hybridization. Bar, 0.1 mm.

Picture from Suzuki Y et al. (2006) Genes Dev "Genetic redundancy masks diverse functions of the tumor suppressor gene PTEN ...."

daf-18 mRNA is present in the germ line. Animals were examined using in situ hybridization. Bar, 0.1 mm.

Picture from Suzuki Y et al. (1999) Development "A BMP homolog acts as a dose-dependent regulator of body size and male tail ...."

Figure 5. Expression pattern of dbl-1::gfp reporter transgenes. All photographs are oriented anterior-left and ventral-down except (E) and (F), which are ventral views. Left panels, Nomarski and right panels, fluorescence images. (A,B) An L4 hermaphrodite. Note the line of dbl-1-expressing neurons in the ventral nerve cord. Arrowhead indicates CANL. (C,D) Head region of an L4 hermaphrodite. Cells tentatively identified as anterior muscle cells (arrowheads) and pharyngeal neurons express dbl-1::gfp. (E,F) Ventral view of posterior trunk (P9.p-P10.p region). Cell bodies of five neurons are seen to express the reporter. The axon bundle of the ventral cord is also visible due to the accumulation of cytoplasmic GFP from the reporter lacking a nuclear localization signal. Arrowheads indicate the cell bodies of the DB7 and DA7 neurons, which send processes circumferentially to the dorsal nerve cord. (G,H) An L4 male tail undergoing morphogenesis. Arrowheads indicate expression of dbl-1::gfp in the spicule socket cells along developing spicules. Another dorsally located expressing cell, seen out of focus, is the DVA neuron.

Picture from Suzuki Y et al. (2002) Genetics "A cuticle collagen encoded by the lon-3 gene may be a target of TGF-beta signaling ...."

Expression patterns of lon-3::gfp reporter transgenes. Integrated lines carrying lon-3::gfp reporter genes were generated as described in materials and methods. (A) L4 hermaphrodite carrying a transcriptional reporter gene with an SV40 nuclear localization signal (pYSL3G1) as an integrated array (kuIs56). GFP is localized to the nuclei of hypodermal cells; many are out of focus. Asterisks indicate nuclei of lateral hypodermal cells (seam cells). (B) Head region of an adult hermaphrodite carrying a translational fusion construct (pYSL3G3) as an integrated array (kuIs55). Note the GFP expression outlining the animal.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.