Picture from Penkner AM et al. (2009) Cell "Meiotic chromosome homology search involves modifications of the nuclear ...."

Figure 1. SUN-1 Forms Aggregates at the Nuclear Envelope during the Time of Ongoing Homolog Pairing (A) A mtf-1/sun-1::gfp;mtf-1/sun-1(ok1282) hermaphrodite gonad expressing SUN-1::GFP (middle, green in merge) was stained for DNA with DAPI (top, blue in merge). (B) SUN-1 staining (iii, red in merge) of hermaphrodite gonads expressing ZYG-12ABC::GFP (ii, green in merge). i, DAPI; iv, merge. The yellow arrowhead marks the position of a patch, while the yellow arrow highlights a focus. (C) DAPI staining (i, iii) and antibody staining (ii, iv). PC-binding proteins HIM-8 or ZIM-3 (red) are shown in combination with SUN-1::GFP (green). The green channel is shifted to the right to allow better visualization of green and red signals. (D) Centrosome marked by SPD-5 staining (red) in mitotic (i) and meiotic (ii, transition zone) nuclei expressing SUN-1:: GFP (green). Scale bars represent 10 um. (E) Western blot to show the in vivo interaction of ZYG12ABC::GFPand endogenous SUN-1 (54 kD, red arrowheads). Coimmunoprecipitations were performed with antiGFP antibodies. SUN-1 was detected with anti-SUN-1 antibodies. Genotypes are on the right.

Picture from Penkner AM et al. (2009) Cell "Meiotic chromosome homology search involves modifications of the nuclear ...."

Figure 1. SUN-1 Forms Aggregates at the Nuclear Envelope during the Time of Ongoing Homolog Pairing (A) A mtf-1/sun-1::gfp;mtf-1/sun-1(ok1282) hermaphrodite gonad expressing SUN-1::GFP (middle, green in merge) was stained for DNA with DAPI (top, blue in merge). (B) SUN-1 staining (iii, red in merge) of hermaphrodite gonads expressing ZYG-12ABC::GFP (ii, green in merge). i, DAPI; iv, merge. The yellow arrowhead marks the position of a patch, while the yellow arrow highlights a focus. (C) DAPI staining (i, iii) and antibody staining (ii, iv). PC-binding proteins HIM-8 or ZIM-3 (red) are shown in combination with SUN-1::GFP (green). The green channel is shifted to the right to allow better visualization of green and red signals. (D) Centrosome marked by SPD-5 staining (red) in mitotic (i) and meiotic (ii, transition zone) nuclei expressing SUN-1:: GFP (green). Scale bars represent 10 um. (E) Western blot to show the in vivo interaction of ZYG12ABC::GFPand endogenous SUN-1 (54 kD, red arrowheads). Coimmunoprecipitations were performed with antiGFP antibodies. SUN-1 was detected with anti-SUN-1 antibodies. Genotypes are on the right.

Picture from Reichman R et al. (2018) Genetics "Mitotic and Meiotic Functions for the SUMOylation Pathway in ...."

D) 3XFLAG::UBC-9 and SUN-1 colocalize the germline.3XFLAG::UBC-9 and SUN-1 images were taken in the pre-meiotic tip, transition zone, andmid/late pachytene. Colocalization is enhanced in the TZ and MLP.

Picture from Sun Y et al. (2013) PLoS One "The F-box protein MEC-15 (FBXW9) promotes synaptic transmission in GABAergic ...."

(B) Representative fluorescence images of young adults expressing GFP under the control of the mec-15 promoter (Pmec-15::GFP, top panel) and mCherry in GABAergic motor neurons (Punc-25::mCherry, middle panel). Co-expression of GFP and mCherry indicates expression of mec-15 in GABAergic motor neurons (cell bodies are labeled with white arrowheads in the merged image). Expression of GFP alone indicates expression of mec- 15 in cholinergic motor neurons (three such cell bodies are labeled with blue arrows). Scale bar is 20 um.

Picture from Sun Y et al. (2013) PLoS One "The F-box protein MEC-15 (FBXW9) promotes synaptic transmission in GABAergic ...."

Figure 1. Behavioral defects in mec-15 mutants and expression of mec-15. (A) mec-15 mutants paralyze faster than wild-type control animals in the aldicarb assay. Paralysis of 20 animals of the indicated genotypes was scored over 2 hours. Fast paralysis of mec-15 mutants is rescued by expressing mec-15 specifically in GABAergic neurons (rescue). Data are averages from 3 independent experiments +/2 standard error of the mean (SEM). (B) Representative fluorescence images of young adults expressing GFP under the control of the mec-15 promoter (Pmec-15::GFP, top panel) and mCherry in GABAergic motor neurons (Punc-25::mCherry, middle panel). Co-expression of GFP and mCherry indicates expression of mec-15 in GABAergic motor neurons (cell bodies are labeled with white arrowheads in the merged image). Expression of GFP alone indicates expression of mec- 15 in cholinergic motor neurons (three such cell bodies are labeled with blue arrows). Scale bar is 20 um.

Picture from Minn I et al. (2009) Mol Biol Cell "SUN-1 and ZYG-12, mediators of centrosome-nucleus attachment, are a functional ...."

Figure 2. C termini of ZYG-12 and SUN-1 are oriented toward thelumen of the nuclear envelope. (A) Diagrams of SUN-1 and ZYG-12.TM, transmembrane domain; CC, coiled-coil domain; K, KASHdomain. Black bar indicates C-terminal 242-amino acid fragment ofSUN-1 and N-terminal 236-amino acid fragment of ZYG-12 used forraising the -SUN-1 and -ZYG-12 polyclonal antibodies, respectively.Numbers represent the amino acid positions of predicteddomains. (B) Fluorescence protease protection assay using syncytialgonads from animals expressing YFP::LMN-1 (qaIs3502[unc-119() pie-1::YFP::LMN-1 pie-1::CFP::H2B]). Immunostaining was performedusing -GFP mAb 3E6 (Invitrogen) to detect YFP::LMN-1and -SUN-1 antibody (Fig. 2 A) to detect SUN-1. Signals fromLMN-1 after trypsin treatment were captured using 30-fold longerexposure relative to those without trypsin treatment. Bar, 10 um. (C)Fluorescence protease protection assay using syncytial gonads fromwild-type animals. Signals from ZYG-12 after trypsin treatmentwere captured using 20-fold longer exposure relative to those withouttrypsin. Bar, 10 um.

Picture from Cuppen E et al. (2003) Comp Funct Genomics "Proteins interacting with Caenorhabditis elegans Galpha subunits."

Expression pattern of Rap1GAP (F) as determined by promoter-GFP reporter constructs. (F) Expression of Rap1GAP in hypodermal cells (hd).

Picture from Li LB et al. (2016) Curr Biol "The Neuronal Kinesin UNC-104/KIF1A Is a Key Regulator of Synaptic Aging and ...."

(A-F) Confocal images of UNC-104::mCherry in the dorsal and ventral nerve cord, which are labeled with myrGFP (D-F), in young (A and D) andaged (B, C, E, and F) worms.

Picture from Vazquez-Manrique RP et al. (2007) Genomics "The frataxin-encoding operon of Caenorhabditis elegans shows complex ...."

(d-f) ptp-2::gfp produced fluorescence in some head neurons (d), gut and spermatheca (e), and body wall muscle (f).

Picture from David DC et al. (2010) PLoS Biol "Widespread protein aggregation as an inherent part of aging in C. ...."

Pkin-19::kin-19::tagrfp animals (F and G).