Picture from Sullenberger, Matthew T et al. (2018) MicroPubl Biol

Figure 1. (A) Schematic of tetra-primer ARMS-PCR with lin-65(n3441) SNP indicated in white, WT SNP in yellow, and mismatches in red. (B) Three replicates of single-worm PCR products of C. elegans gene lin-65 in N2 strain, a strain homozygous for the lin-65(n3441) SNP allele, and heterozygous (n3441/WT) F1 progeny under standard PCR conditions (left) and conditions optimized for amplification of the SNP flanking sequence (right). (C) PCR products of parental, F1, and F2 individuals with flanking sequence amplification.