Picture from Cook, Steven J. et al. (2021) MicroPubl Biol

Figure 1. AVH and AVJ neurons and hlh-34 expression: A: AVH and AVJ display a nearly identical position and axon trajectory in the lateral ganglion of C. elegans. Images used with permission from Wormatlas. B: Whole-animal lateral and dorsoventral overviews of hlh-34prom::gfp (otIs768 transgene) expressing young larvae. Animals imaged in a NeuroPAL (otIs669) background with only GFP emission shown for simplicity. C: Expression pattern of otIs768 and sIs14542 transgenic reporters in a NeuroPAL (otIs669) background. GFP signal is only observed in the AVH neuron. D: Embryonic expression pattern of otIs768. At ~360 minutes the GFP signal is observed in the AVH neuron and its dying sibling cell. All animals were imaged in a NeuroPAL (otIs669) background with only GFP emission shown for simplicity. E: Lineages of AVH/J in red. CEPsoDL/R shown in orange. Dying siblings of AVH shown as 'x'. F: hlh-34 locus and promoter fragment used in the sIs14542 transgene. Coordinates are V:13709947..13712813 in WS140 and V:13707915..13707382 in WS280. The size of the promoter used in the leEx1692 strain, kindly provided by Ian Hope, could not be unambiguously determined. The strain used in the first publication on hlh-34 (Cunningham et al. 2012) used 2.5kb upstream of hlh-34. G: Heatmap of scRNA expression (Taylor et al. 2021), showing hlh-34 expression and other markers of AVH and AVJ identity.

Picture from Steven R et al. (2005) Genes Dev "The UNC-73/Trio RhoGEF-2 domain is required in separate isoforms for the ...."

Figure 4. Expression patterns of the unc-73 isoforms. The patterns of expression of the unc-73 isoforms B through F were determined using constructs that each specifically encode an individual unc-73 isoform tagged with GFP at the C terminus. The constructs were injected into wild-type animals, and the larvae and adults of the resulting stable lines were examined. All animals are oriented with the posterior to the left and anterior to the right. (A) UNC-73 B is strongly expressed in the axons of neurons. A cross-sectional side view of a late-larval stage animal reveals the nerve ring as two bright patches of fluorescence around the pharynx (arrowheads). (B) Expression of D1 driven by the myo-2 promoter in [myo-2p::D1::GFP] ev802/unc-11 dpy-5 animals is limited to the muscles of the pharynx. The C1 isoform is expressed in a limited set of neurons in the midbody of the animal (C) and the head (D). Arrowheads indicate the ventral cord. Also visible in the head are the neuronal support socket and/or sheath cells. The C2/F isoform has the same pattern of expression as C1. (E) D1 expression in the muscles of the pharynx and two neurons in the head. A few unidentified axons are faintly visible in a cross-section of the nerve ring (arrowheads). (F) The processes of D1-expressing neurons are visible in the ventral cord. The muscles of the vulva seen in the shape of an X, and the HSNR cell body (arrowhead) also express D1. The D2 isoform has the same pattern of expression as D1. (G) UNC-73 E is expressed in the axons and cell bodies of most if not all neurons and the excretory canals. The bright region in the head is a result of expression in axons of the nerve ring and the cell bodies of neurons in the ganglia surrounding the nerve ring.

Picture from Steven RM et al. (1998) Cell "UNC-73 activates the Rac GTPase and is required for cell and growth cone ...."

Figure 4. UNC-73 Expression Pattern. (A) At the beginning of neurulation (comma stage embryo), expression of unc-73/gfp is strongest in the region of the developing nervous system in the head (white arrowheads) and in the ventral nerve cord (yellow arrows).(B) The P cells (white arrowheads) begin to express in early L1 just before they intercalate on the ventral midline. The process of intercalation proceeds from anterior to posterior (left to right) and is paralleled by a progressive anterior to posterior increase in expression of the unc-73/gfp reporter.(C and D) In mid-L1, expression comes on in the Q neuroblasts on the left and right (white arrowheads in C and D, respectively). In this animal, QL has migrated posteriorly and has yet to divide, and QR has migrated anteriorly and divided once.(E) The honeycomb expression in this L4 animal is most likely the somatic gonad. The distal tip cell (yellow arrow) at the leading edge of the growing somatic gonad and the excretory canal (thick blue arrows) also express unc-73/gfp. The dorsal nerve cord (white arrowheads) is also visible. Red arrows in (B), (C), and (F) point to CAN neurons. The transgenes in the animals of (A) and (F) were extrachromosomal and may have been lost in some cells.(F) The motorneurons in the ventral nerve cord (e.g., white arrowheads) express throughout postembryonic development. The HSNL neuron (thick yellow arrow) and spindle-shaped body muscles are also visible.(G) A wild-type L3 animal stained with an affinity purified polyclonal antibody to UNC-73. Note intense staining of nerve ring (yellow arrow) and ventral nerve cord (white arrowheads).Scale bars = 10 μm.

Picture from Reilly DK et al. (2019) MicroPubl Biol "Evolution of hermaphroditism decreases efficacy of Ascaroside#8-mediated ...."

Figure 1 Caenorhabditis species males respond differentially to ascr8. (A) Dwell times of Caenorhabditismales in vehicle control (-) and ascr8 (+), respectively. Hermaphroditic species were tested alongside theirgonochoristic sister-species (denoted by dotted horizontal brackets: C. elegans vs. C. inopinata, C. briggsae vs. C.nigoni, C. tropicalis vs. C. wallacei). The Japonica Group species, C. japonica and C. afra, (left of vertical dottedline) were also tested for their attractive response. Species are displayed according to the most recent phylogeneticanalysis of the Caenorhabditis genus (Stevens et al., 2019). No species were as attracted to ascr8 as C. elegans,although C. briggsae, C. nigoni, and C. wallacei did spend significantly more time in ascr8 compared to the vehicle. Error bars denote SEM. n 12. Paired t-tests or Wilcoxon test (dependent on Normality test) of ascr8 vs.vehicle control. (B) The Log2 of Transformed fold-change of ascr8 vs vehicle dwell times. See Description for transformation calculations. Among the sister-species pairs, gonochoristic species exhibited a significant increase inascaroside dwell time. Student's t-test. Brackets linking species denote sister-species pairs, with androdioeciousspecies on the left, gonochoristic on the right. * p < 0.05, **** p < 0.0001.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.

Picture from Millonigg S et al. (2014) PLoS Genet "GLD-4-mediated translational activation regulates the size of the ...."

(A) GLD-4 expression is equal across the distal germ line. GLD-2 intensities increase from low-to-high in a distal-to-proximal manner. Extruded gonads of indicated genotype stained with DAPI, a-GLD-2, a-GLD-4, and a-GLH-2 as a positive tissue penetration control (not shown). Asterisk, distal tip; arrowhead, mitosis-to-meiosis boundary.