Figure 2. Expression pattern and sub-cellular localization of MAGI-1::GFP. a, expression of the rescuing
magi-1::gfp construct in the head of an adult worm visualized by fluorescence confocal microscopy. b and c, list of the neurons expressing MAGI-1. MAGI-1 and GLR-1 double positive cells are indicated in bold. Neurons that project their processes along the ventral nerve chord are listed in c. d-f, co-localization of MAGI-1::YFP with the presynaptic marker, synaptobrevin (SNB-1) driven by endogenous promoters. Asterisks label the synaptic area, arrows point to the punctae flanking the synaptic cleft. Scale bars represent 10 µm on panel a, and 1 um on panel f.