Picture from Stein, Samantha A. et al. (2022) MicroPubl Biol

Figure 1. glp-1(bn18) enhancer allele om92 disrupts germline function and is likely a null allele of the ekl-1 gene:(A) DIC images of representative germlines from young adult hermaphrodites of the indicated strains raised at 20 °C. Asterisks indicate the distal tip cell. Arrows indicate location of sperm. Arrowheads indicate location of oocytes. (B) Fluorescence images of representative DAPI-stained germlines from young adult hermaphrodites of the indicated strains raised at 20 °C. Marking symbols are the same as in (A). (C) Screenshot of ekl-1 gene location on LG I and gene organization from Wormbase (WS285, https://wormbase.org) indicating location and length of the ekl-1(om92) deletion. (D) Partial sequence alignment of ekl-1(om92) and ekl-1(+) genomic sequence indicating precise extent and location of the ekl-1(om92) deletion. Exon sequences are uppercase while intron sequences are lowercase. WT, wildtype. (E) Schematic diagram of predicted proteins encoded by ekl-1(om92) (above) and ekl-1(+) (below). Red boxes in the predicted wildtype EKL-1 protein indicate locations of Tudor domains according to UniProt (Release 2022_03, https://www.uniprot.org/). The black box in the predicted mutant EKL-1 protein indicates the extent of the amino acid sequence changes caused by the ekl-1(om92) deletion and resulting frameshift. The C-terminal amino acid sequence resulting from the frameshift (after residue T81) is shown. The predicted length of the mutant EKL-1 protein is 100 amino acids, and the wildtype protein is 606 amino acids.

Picture from Stein KK et al. (2010) Genetics "Functional redundancy of paralogs of an anaphase promoting complex/cyclosome ...."

gfi-3 transcriptional fusion is expressed predominantly in the adult germline and embryo. gfi-3::mCherry::H2B::gfi-3 stable transgenes were imaged by fluorescence microscopy (E-H) and DIC (F and G). gfi-3 (E) adult hermaphrodites exhibit expression in the germline extending from the distal tip (inset) to the -1 oocyte (arrowhead, E). Sperm expression is observed in the spermathecae (arrow, E). Somatic gut nuclei (asterisk, E) also express gfi-3. gfi-3 is expressed at all embryonic stages, including meiosis (arrow, F) and mitosis (G). Male germlines also express the gfi-3 (H) transgene; mature sperm are indicated with an arrow. Bars, 10 um.

Picture from Stein KK et al. (2010) Genetics "Functional redundancy of paralogs of an anaphase promoting complex/cyclosome ...."

such-1 transcriptional fusion is expressed predominantly in the adult germline and embryo. such-1::mCherry::H2B::such-1 and gfi-3::mCherry::H2B::gfi-3 stable transgenes were imaged by fluorescence microscopy (A-D) and DIC (B and C). such-1 (A) adult hermaphrodites exhibit expression in the germline extending from the distal tip (inset) to the -1 oocyte (arrowhead, A). Sperm expression is observed in the spermathecae (arrow, A). such-1 is expressed in meiotic (arrow, B) and mitotic embryos (C). Male germlines also express the such-1 (D) transgene; mature sperm are indicated with an arrow. Bars, 10 um.

Picture from Stein KK et al. (2010) Genetics "Functional redundancy of paralogs of an anaphase promoting complex/cyclosome ...."

Figure 4. such-1 and gfi-3 transcriptional fusions are expressed predominantly in the adult germline and embryo. such-1::mCherry::H2B::such-1 and gfi-3::mCherry::H2B::gfi-3 stable transgenes were imaged by fluorescence microscopy (A-H) and DIC (B, C, F, and G). such-1 (A) and gfi-3 (E) adult hermaphrodites exhibit expression in the germline extending from the distal tip (inset) to the -1 oocyte (arrowhead, A and E). Sperm expression is observed in the spermathecae (arrow, A and E). Somatic gut nuclei (asterisk, E) also express gfi-3. such-1 is expressed in meiotic (arrow, B) and mitotic embryos (C). gfi-3 is expressed at all embryonic stages, including meiosis (arrow, F) and mitosis (G). Male germlines also express the such-1 (D) and gfi-3 (H) transgenes; mature sperm are indicated with an arrow. Bars, 10 um.

Picture from Wenick AS et al. (2004) Dev Cell "Genomic cis-regulatory architecture and trans-acting regulators of a single ...."

Figure 7. AIY-Expressed Genes(A) Classification of AIY-expressed genes. 154 top-scoring genes from the two AIY hit lists (high-score and low-mismatch) and 100 genes ranking 10,000 were annotated and classified as shown. The 'others' class is further subdivided as shown in the Supplemental Tables.Numbers for the genome-wide annotation were extracted from Stein et al. (2003), Bargmann (1998), and Nathoo et al. (2001). Note that the under representation of putative gene-regulatory factors and metabolism/homeostasis genes in the AIY gene battery is expected from targets of terminally acting transcription factors that determine the subtype identity of a cell, but not general properties of the cell.(B) Neurotransmitter receptors that contain AIY motifs in the cis-regulatory regions. Shading in gray indicates that AIY expression was confirmed by analyzing gfp reporter fusions ([C] and Supplemental Figure S7). Note that the AIY-expressed neuropeptide receptors are not enriched for any specific receptor subtype. Percentages indicate number of genes predicted to be expressed in AIY compared to the total number of genes in the genome.(C) Examples of selected neurotransmitter receptor gfp fusions. Upper panels show complete worms, lower panels show the head region with AIY expression indicated in yellow due to an overlap of the gfp signal with the AIY-specific rfp reporter transgene otIs133. The complete set of gfp reporters is shown in Supplemental Figure S7.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.