Picture from O'rourke SM et al. (2007) PLoS Genet "Dynein modifiers in C. elegans: light chains suppress conditional heavy chain ...."

Localization of GFP-Tagged Dynein Suppressor Proteins in Wild-Type Embryos. STAR-2 (a nonspecific suppressor gene): apparent localization to germline P-granules.

Picture from Shen C et al. (2005) J Biol Chem "Roles of the HIF-1 hypoxia-inducible factor during hypoxia response in ...."

Figure 4. phy-2:GFP expression in an adult hermaphrodite. The positions of the vulva and the spermathecas are indicated with a star and an s, respectively.

Picture from Hyenne V et al. (2015) J Cell Biol "RAL-1 controls multivesicular body biogenesis and exosome secretion."

(E-H) APEX::RAL-1(DN) shows DAB staining both at apical membrane stacks (E and F, arrowheads) and at the external surface of MVBs (E and G-G'', arrows). Animals expressing no APEX tag treated with DAB show no staining (H). The star indicates a MVB. APM, apical plasma membrane.

Picture from Morin, Marie-Charlotte et al. MicroPubl Biol

Figure 1: PCR-based detection of homozygous wild type and mutant alleles for mab-9. (A) primers design allowing the introduction of an Acc65I site in the PCR product from the mutant e2410 allele. Blue nucleotides: sequence of the primers used; Red nucleotides: DNA alterations in the e2410 allele; Green nucleotide: PCR-introduced change. Small lettering, intronic sequence; capitals, exons. (B) Representative analysis on a 2,5% agarose gel. Lane L: ladder, the 500pb band is indicated; lanes 1: WT allele; lanes 2: mutant allele; lane 3: heterozygote animal. The mab-9 fragment amplified from the WT allele is left undigested by Acc65I with a size of 358pb (white star), while the PCR fragment length obtained from mutant e2410 allele is 279pb after Acc65I digestion (red star, the remaining 79pb fragment is not visible here).

Picture from Janssen T et al. (2008) Endocrinology "Discovery of a cholecystokinin-gastrin-like signaling system in ...."

FIG. 7. Expression pattern of C. elegans nlp-12. Confocal images of transgenic wild-type N2 worms (adult) expressing mCherry under the control of the nlp-12 promoter and internal regulatory sequences. Head section (A) with the posterior pharyngeal bulbus (B) middle section with the vulva (C) tail section. White arrows point to the ccs, and white arrowheads indicate the DVA tail neuron and its anterior process. The white star indicates autofluorescence of the gut.

Picture from Hyenne V et al. (2015) J Cell Biol "RAL-1 controls multivesicular body biogenesis and exosome secretion."

Figure 2. RAL-1 localizes at the surface of MVBs. (A-D) WT (RAL-1(WT); A) and dominant negative (RAL-1(DN); C) versions of RAL-1, but not the constitutively active version (RAL-1(CA); B), colocalize fully with VHA-5 in the epidermis at the time of alae formation, as shown in the quantification (D). In D, the numbers inside the bars indicate the number of puncta (number of animals). (E-H) APEX::RAL-1(DN) shows DAB staining both at apical membrane stacks (E and F, arrowheads) and at the external surface of MVBs (E and G-G'', arrows). Animals expressing no APEX tag treated with DAB show no staining (H). The star indicates a MVB. APM, apical plasma membrane.

Picture from Gupta BP et al. (2003) Development "The C. elegans LIM homeobox gene lin-11 specifies multiple cell fates during ...."

Figure 4. Developmental expression of lin-11::GFP (syIs80) in vulval cells. (A,C,E) Nomarski photographs. (B,D,F) lin-11::GFP-expressing vulval cells marked with arrows. Few VC neurons are also visible (star). (A,B) During Pn.px stage, weak GFP fluorescence is detected in the P5.pp and P7.pa cells (arrows in B). (C,D) By Pn.pxx stage, vulval cells express high levels of GFP. The VPC lineage tree has been drawn. In this animal P5.ppx, P6.ppx and P7.pax cells are seen expressing GFP. (E,F) During L4 stage, Pn.pxxx cells express GFP in the P5.p and P7.p progeny. The region occupied by each VPC progeny has been marked. In this focal plane, only a subset of the cells is visible (arrows). Anterior is towards the left. Scale bar: 10 um.

Picture from Haklai-Topper L et al. (2010) Gene Expr Patterns "The neurexin superfamily of Caenorhabditis elegans."

Fig. 3. itx-1 expression. (A): itx-1prom::gfp expression in non-neuronal cells. (a) Intestinal expression in embryo and (b) in an L1 larva. (c) expression in spermatheca (white arrows) and (d) in vulva (white arrows). Blue star in (c) and (d) indicates gut expression. (B): itx-1prom::gfp expression in glial cells. (a) Expression in amphid socket cells; gfp signal is inverted for contrast; (b-e) co-labeling of itx-1-expressing sheath cells and sensory neurons filled with dye, as previously described (Perkins et al., 1986) (b), socket cells with itx-1prom::gfp (c), Normarksi image (d) and overlap of red and green in (e); (f): Sensory anatomy with associated sheath cell nuclei labeled in blue, socket cell nuclei in green and sensory neuron nuclei in red. Adapted from (Ward et al., 1975).

Picture from Pirri JK et al. (2009) Neuron "A tyramine-gated chloride channel coordinates distinct motor programs of a ...."

Figure 4. Expression Pattern of lgc-55. (A) Adult animal showing expression of a fluorescent lgc-55::mCherry (zfIs4) transcriptional reporter in neck muscles, head neurons, SDQ, HSN, and ALN neurons.(B) Head region of an adult animal showing expression of lgc-55::mCherry in head neurons, IL1, RMD, SMD, and AVB.(C) Expression of lgc-55::GFP (zfIs6) in the HSN and uterine UV1 cells. The star indicates position of the vulva.(D) Expression of lgc-55::GFP in the third row of neck muscle cells.(E) Expression of LGC-55::GFP (zfEx38) translational reporter showing subcellular localization to neuronal cell bodies, nerve ring and muscle arms. Position of the nerve ring is indicated by the dotted line. Scale bar, 10 μm. Anterior is to the left and ventral side is down in all images.

Picture from Warner A et al. (2011) Mol Biol Cell "The Caenorhabditis elegans paxillin orthologue, PXL-1, is required for ...."

Figure 9: Actin attachment complexes in pharyngeal muscle. Paxillin is localized to both full and partial ring-like structures in pharyngeal muscle. Full and partial ring-like PXL-1 localization using an anti-GFP antibody (A), along with punctate actin localization (B) using an anti actin antibody. (C) Actin at the middle of the PXL-1 ring-like pattern, with arrows indicating podosome-like attachment structures. The PXL-1 localization in pharyngeal muscle using an anti-GFP antibody (D) is identical to that of DEB-1/vinculin antibody staining (E), as shown in the overlay (F) of their localization patterns. The luminal face of the pharyngeal muscle (G) contains attachment structures (arrows) that differ in size and shape from those found in the cuticular face (H) as shown by TEM. Filaments can be seen projecting into the muscle cell from each type of attachment, as indicated by arrowheads. Membrane invaginations are also visible at the cuticular face of the muscle cell as marked by a star. Bar, 1 um.