Picture from Srinivasan S et al. (2008) Cell Metab "Serotonin regulates C. elegans fat and feeding through independent molecular ...."

Expression Patterns of ech-3. Inset panel shows the corresponding differential interference contrast image.

Picture from Srinivasan S et al. (2008) Cell Metab "Serotonin regulates C. elegans fat and feeding through independent molecular ...."

Figure 4. Expression Patterns of b-Oxidation Genes (A-H) Representative images of transgenic animals expressing GFP reporter fusions for promoters of the indicated genes. Inset panels show corresponding differential interference contrast images. (I) Change in transcript levels of the indicated metabolic genes upon 5-HT treatment in wild-type animals as determined by real-time qPCR. Data are reported as the average of two independent cDNA preparations +/- SEM.

Picture from Srinivasan S et al. (2008) Cell Metab "Serotonin regulates C. elegans fat and feeding through independent molecular ...."

Expression Patterns of acs-5. Inset panel shows the corresponding differential interference contrast image.

Picture from Srinivasan DG et al. (2003) Genes Dev "A complex of LIN-5 and GPR proteins regulates G protein signaling and spindle ...."

DNA staining with DAPI (blue) and immunostaining of GPR-1/GPR-2 (green) and LIN-5 (red). Merged images are shown in D, H, L, and P. (A-D) Fertilized embryo in meiosis II. LIN-5, but not GPR-1/GPR-2, showed specific localization to the meiotic spindle (arrowheads, B,C). (E-H) One-cell embryo in metaphase. GPR-1/GPR-2 (F) and LIN-5 (G) colocalize to the spindle asters (arrowheads) and kinetochore MTs (arrows). (I-L) Two-cell embryo. GPR-1/GPR-2 (J) and LIN-5 (K) colocalize to the spindle apparatus as well as the cell cortex (arrowheads). (M-P) Four-cell embryo. In addition to spindle staining, both proteins showed stronger staining at the P2/EMS boundary (arrowhead) than at other cell membranes (arrow). Bar, 10 μm.

Picture from Srinivasan S et al. (2008) Cell Metab "Serotonin regulates C. elegans fat and feeding through independent molecular ...."

Expression Patterns of EEED8.2. Inset panel shows the corresponding differential interference contrast image.

Picture from Srinivasan S et al. (2008) Cell Metab "Serotonin regulates C. elegans fat and feeding through independent molecular ...."

Expression Patterns of acdh-10. Inset panel shows the corresponding differential interference contrast image.

Picture from Srinivasan DG et al. (2003) Genes Dev "A complex of LIN-5 and GPR proteins regulates G protein signaling and spindle ...."

Figure 3. GPR-1/GPR-2 localize to the mitotic spindle and cell cortex in a LIN-5-dependent manner. DNA staining with DAPI (blue) and immunostaining of GPR-1/GPR-2 (green) and LIN-5 (red). Merged images are shown in D, H, L, P,T, and X. (A-D) Fertilized embryo in meiosis II. LIN-5, but not GPR-1/GPR-2, showed specific localization to the meiotic spindle (arrowheads, B,C). (E-H) One-cell embryo in metaphase. GPR-1/GPR-2 (F) and LIN-5 (G) colocalize to the spindle asters (arrowheads) and kinetochore MTs (arrows). (I-L) Two-cell embryo. GPR-1/GPR-2 (J) and LIN-5 (K) colocalize to the spindle apparatus as well as the cell cortex (arrowheads). (M-P) Four-cell embryo. In addition to spindle staining, both proteins showed stronger staining at the P2/EMS boundary (arrowhead) than at other cell membranes (arrow). (Q-X) Localization in RNAi embryos. (Q-T) GPR-1/GPR-2 staining is strongly reduced but not fully eliminated in gpr-1/gpr-2(RNAi) embryos. (S) LIN-5 staining remained at the spindle apparatus but appeared reduced at the cell periphery. (U-X)lin-5 RNAi eliminated LIN-5 staining (W) and disrupted GPR-1/GPR-2 localization (V). Bar, 10 μm.

Picture from Srinivasan S et al. (2008) Cell Metab "Serotonin regulates C. elegans fat and feeding through independent molecular ...."

Expression Patterns of F08A8.4. Inset panel shows the corresponding differential interference contrast image.

Picture from Srinivasan S et al. (2008) Cell Metab "Serotonin regulates C. elegans fat and feeding through independent molecular ...."

Expression Patterns of F01G4.2. Inset panel shows the corresponding differential interference contrast image.

Picture from Srinivasan S et al. (2008) Cell Metab "Serotonin regulates C. elegans fat and feeding through independent molecular ...."

Expression Pattern of W01A11.5. Inset panel shows the corresponding differential interference contrast image.