Picture from Nehrke K et al. (2002) J Biol Chem "The NHX family of Na+-H+ exchangers in Caenorhabditis elegans."

Q and R, the nhx-9 promoter drove GFP expression in the excretory cell (ec) as well as in the phasmid sensory organs in the tail (phm) and the posterior intestine (int; AI and AJ).

Picture from Hsu TY et al. (2010) Curr Biol "Engulfment of apoptotic cells in C. elegans is mediated by integrin alpha/SRC ...."

(Aa-Aj) INA-1::GFP is enriched in the pseudopods extending around apoptotic cells. Time-lapse images of INA-1::GFP (Aa-Ae) and differential interference contrast (DIC; Af-Aj) during cell corpse internalization in an embryo expressing Pced-1ina-1::gfp are shown. 0 min indicates the time point at which INA-1::GFP was first seen to accumulate at the contact site with C3. Arrowheads indicate the apoptotic cell (C3); arrows indicate the engulfing cell (ABplaapppp). Scale bar in (Aa) represents 5 um.

Picture from Kazatskaya A et al. (2017) Genetics "Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the ...."

(B) Representative images of the indicated GFP-tagged MAPK-15 reporters in wild-type worms. Constructs (shown in schematicsat right) derived from mapk-15 genomic sequences (red) under the control of mapk-15 upstream regulatory promoter sequences(Prom; orange). Strains expressing the mapk-15a::gfp DNA transgene at low levels generated by injecting worms with a low DNAconcentration (0.2 ng/ul). BB: basal body. AJ: apical junction. Cil: ciliary axoneme. Anterior is at top (head images) and left (tailimages). Scale bars: 3 um (head images) and 5 um (tail images).(C) Representative images of localization of GFP::MAPK-15c expressed under its endogenous promoter in mapk-15(gk1234)animals. BB: basal body. AJ: apical junction. Cil: ciliary axoneme. Anterior is at top (head images) and left (tail images). Scale bars:5 um.

Picture from Kazatskaya A et al. (2017) Genetics "Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the ...."

Figure S2. GFP-tagged MAPK-15 localizes to the cilia and ciliary base, and mapk-15 expression in most ciliated neurons isDAF-19-independent.(A) Representative images of mapk-15p::gfp expression in wild type (WT) and daf-19(m86);daf-12(sa204) worms. Lab: inner labialcells, quad: outer labial quadrant cells. Schematic of the mapk-15p::gfp reporter construct shows mapk-15 upstream regulatorysequences (+ first 11 bp of exon 1) fused to gfp, and the location of a putative X-box sequence. Numbers refer to genomic positionsrelative to the start codon. Anterior is at left. Scale bars: 20 um.(B) Representative images of the indicated GFP-tagged MAPK-15 reporters in wild-type worms. Constructs (shown in schematicsat right) derived from mapk-15 genomic sequences (red) under the control of mapk-15 upstream regulatory promoter sequences(Prom; orange). Strains expressing the mapk-15a::gfp DNA transgene at low levels generated by injecting worms with a low DNAconcentration (0.2 ng/ul). BB: basal body. AJ: apical junction. Cil: ciliary axoneme. Anterior is at top (head images) and left (tailimages). Scale bars: 3 um (head images) and 5 um (tail images).(C) Representative images of localization of GFP::MAPK-15c expressed under its endogenous promoter in mapk-15(gk1234)animals. BB: basal body. AJ: apical junction. Cil: ciliary axoneme. Anterior is at top (head images) and left (tail images). Scale bars:5 um.

Picture from Hsu TY et al. (2010) Curr Biol "Engulfment of apoptotic cells in C. elegans is mediated by integrin alpha/SRC ...."

Figure 2. INA-1 Recognizes Apoptotic Cells and Is Required for Their Internalization by Engulfing Cells. (Aa-Aj) INA-1::GFP is enriched in the pseudopods extending around apoptotic cells. Time-lapse images of INA-1::GFP (Aa-Ae) and differential interference contrast (DIC; Af-Aj) during cell corpse internalization in an embryo expressing Pced-1ina-1::gfp are shown. 0 min indicates the time point at which INA-1::GFP was first seen to accumulate at the contact site with C3. Arrowheads indicate the apoptotic cell (C3); arrows indicate the engulfing cell (ABplaapppp). Scale bar in (Aa) represents 5 μm.(Ba-Bc) ina-1 mutations reduce the efficiency of cell corpse internalization in engulfing cells.(Ba and Bb) MYRI::GFP and DIC images of wild-type (Ba) and ina-1(gm144) (Bb) embryos expressing Pced-1myri::gfp. Arrows indicate apoptotic cells. Scale bar in (Ba) represents 5 μm.(Bc) Percentage of apoptotic cells with a MYRI::GFP circle in the indicated genotype. Data (mean ± SD) are the average for two independent transgenic lines. Numbers in parentheses indicate the number of cell corpses scored.(Ca-Cc) INA-1(N) binds to the surface of apoptotic cells in a scrm-1-dependent manner.(Ca and Cb) INA-1(N)::GFP and DIC images of ced-1(e1735); ced-5(n1812) (Ca) and scrm-1(RNAi)ced-1(e1735); ced-5(n1812) (Cb) embryos expressing Phspina-1(N)::gfp. Arrows indicate apoptotic cells. Scale bar in (Ca) represents 5 μm.(Cc) Percentage of INA-1(N)::GFP+ cell corpses in the indicated genotype. Cell corpses fully enclosed by the INA-1(N)::GFP circle were scored as INA-1(N)::GFP+. Each sample point represents the data from 12 embryos. Data are shown as mean ± SD.

Picture from Kitaoka S et al. (2013) PLoS One "FGT-1 is a mammalian GLUT2-like facilitative glucose transporter in ...."

Figure 5. Cellular and subcellular localizations of FGT-1 in C. elegans. The fgt-1::egfp fusion construct under the control of the fgt-1 promoter was injected into C. elegans, and the expression of the FGT-1::eGFP fusion protein was visualized with a confocal laser microscope. A: whole animal, B: embryos inside of a parental worm, C: higher magnification image of mid-body. For each panel (A to C), GFP fluorescence was shown alone or as a merged picture with differential interference contrast (DIC) images. D-H. Immunostaining of the apical membrane marker IFB-2 for fgt-1::egfp embryos. Subcellular localizations of FGT-1::eGFP (green) and IFB-2 (red) were observed under a confocal laser microscope. D: FGT-1::eGFP, E: IFB-1 immunostaining. F: Merged image of D and E.G: The higher magnification of the white box area in F.H: Merged image of eGFP fluorescence and DIC images. I. Schematic diagram of an intestinal cell with subcellular localizations of eGFP (green) and IFG-2 (red). The potential adherent junction (AJ) is indicated by a dark box.

Picture from Gobel V et al. (2004) Dev Cell "Lumen morphogenesis in C. elegans requires the membrane-cytoskeleton linker ...."

Figure 1. ERM-1 Is Enriched at Luminal Surfaces. Here and elsewhere, animals are viewed from the side: anterior left, posterior right, dorsal top, ventral bottom. Gray boxes in schematics (iA, iiA, and iiiA) indicate areas magnified in images beneath. (iC and iiB) Nomarski images; (iD and iF) confocal sections; (iE, iiC, iiiB, and iiiC) confocal projections.i. Intestine. (A) ERM-1 localizes to intestinal lumen (green). (B) Wild-type intestine with 20 bilaterally symmetrical cells in 9 rings (4 cells for first ring; blue), intestinal lumen (green), AJ (red); tube is rotated along longitudinal axis. (C-F) Anterior intestinal tube; apical (small arrowheads) and basal (large arrowheads) sides of tube indicated. ERM-1-GFP (D and E) is at the lumen, apical to but distinct from α-AJM-1 (yellow in [E] from integration of sections, not overlap). ERM-1-GFP also seen in excretory canal in (E) (arrow). NFM-1-lacZ (F) is localized basolaterally.ii. Excretory canals. (A) ERM-1 localizes to excretory canals (red; canals shown separated for clarity). (B and C) Anterior excretory canals: ERM-1-GFP outlines canals and also the intestinal (arrow), but not pharyngeal (arrowhead) lumen.iii. Gonad. (A) ERM-1 localizes to gonad lumina: spermathecae, uterus, vulva (blue). (B) Integration of sections through uterine-vulval connection (ventral view); vulva opens to right: ERM-1-GFP outlines eggs in uterus and vulval opening (arrowhead). (C) Vulva (large arrowhead) at higher magnification (ventrolateral view; vulva opens to right): ERM-1-GFP is separate from complex junctional α-AJM-1 pattern (yellow from integration of sections, not overlap); intestinal lumen at left (small arrowhead).

Picture from Nehrke K et al. (2002) J Biol Chem "The NHX family of Na+-H+ exchangers in Caenorhabditis elegans."

Figure 2. GFP expression driven by 4-kb nhx gene promoter fragments in transgenic nematodes.Representative fluorescent and the corresponding differential interference contrast photomicrographs are shown side by side for transgenic lines expressing GFP from 4-kb promoter fragments from the nhx gene family. A-H, transgenic C. elegans expressing nhx-1, -2, -7, and-6::GFP, respectively, in the intestine (int). nhx-1 and-2::GFP are expressed throughout the length of the intestine, whereas nhx-7::GFP is expressed only in the most posterior cells; nhx-6::GFP is expressed most strongly in posterior intestinal cells, with a graded level of expression that weakens moving anteriorly then strengthens again in the most anterior cells. The direction that the nematodes are facing is indicated in the differential interference contrast view by an arrow. I and J, nhx-3:: GFP expression in hypodermal cells (hyp). Strong expression of nhx-3::GFP was also observed in the spermathecal junction cells (sjc) and ut1 cells of the uterus (ut) in adult animals (U and V). K and L, strong nhx-4::GFP expression was observed in the nerve ring and retrovesicular ganglia and hypodermal cells associated with the intestinal tract and rectum, but varying levels of expression were observed in all cells of the body (data not shown). Mosaic animals are shown that express nhx-4::GFP in hypodermal cells (hyp) and neurons of an L1 larvae (W and X), the distal tip cell (dtc; Y and Z), and pharyngeal neurons (phx; AA and AB). The nhx-4 promoter drives GFP expression in all of the neurons of the worm (M and N) as well as in the excretory cell (ec; AC and AD), and the ppar and ppal cells of the vulva (vul; AE and AF). AG and AH, a more detailed view of nhx-4::GFP expression in the pharyngeal neurons (phx). O and P, transgenic nematodes expressing nhx-8::GFP in the lateral seam cells (sc). Q and R, the nhx-9 promoter drove GFP expression in the excretory cell (ec) as well as in the phasmid sensory organs in the tail (phm) and the posterior intestine (int; AI and AJ).