Figure 1: Tagged
glp-1 alleles are expressed in the distal germline, embryo, and spermatheca. (A) Schematic of tagged
glp-1 alleles. (B) Rescue of
glp-1 null phenotype by MosSCI
glp-1 alleles. (C-F) Adult hermaphrodite gonads, oriented distal to the left. Arrow, spermatheca. (F) Arrowhead, GLP-1::6xMyc6xHis visible within spermathecal cell nucleus. Staining in oocyte nuclei likely includes a large component of background staining because staining is present at similar levels in controls. (G) Adult male gonads. (C-G) Dashed line, outline of gonad. (H) Embryo expressing GLP-1::4xV5. Images are maximum-intensity z-projections through equal z-ranges of the same embryo. Anti-V5 staining in control embryos (not shown) was uniformly dim. (C-H) Scale bar, 20 um. (I) Distal-most germ cell nuclei of adult hermaphrodite. Dashed line, germ cell nucleus. Arrowhead, GLP-1::6xMyc6xHis visible within germ cell nucleus. Scale bar, 2 um. (C-G, I) Control, animals lacking a tagged
glp-1 allele.