Picture from Singhvi A et al. (2016) Cell "A Glial K/Cl Transporter Controls Neuronal Receptive Ending Shape by Chloride ...."

Figure S6. Some pde Alleles Do Not Affect AFD Shape, Related to Figure 6(A) Histogram details as in Figure 1B.(B) Detail as in Figure S3.(C) Fluorescence image depicting expression of PDE-1 in AFD (dotted line).(D and E) Fluorescence images of wild-type and elongated microvilli, respectively, in animals overexpressing PDE-1B. Yellow dots, wild-type AFD extent. Red dots, elongated portion. Scale bar, 5 um.

Picture from Singhvi A et al. (2008) Genetics "The T-box gene tbx-2, the homeobox gene egl-5 and the asymmetric cell division ...."

Figure 10. Model summarizing genetic data implicating TBX-2, HAM-1, and EGL-5 as regulators of the survival and fate of the precursor cell, the HSN neuron, and the PHB neuron, as well as migration of the HSN neuron. We propose that egl-5 acts to promote HSN development and to inhibit PHB fate in the HSNs. These models are not mutually exclusive. By inhibiting PHB fate, for example, egl-5 could promote the expression of HSN traits.

Picture from Singhvi A et al. (2016) Cell "A Glial K/Cl Transporter Controls Neuronal Receptive Ending Shape by Chloride ...."

(A) GCY-8 localization to AFD microvilli receptive endings. Arrow, AFD microvilli (zoomed in inset). Arrowhead, dendrite.

Picture from Singhvi A et al. (2016) Cell "A Glial K/Cl Transporter Controls Neuronal Receptive Ending Shape by Chloride ...."

(D and E) Arrow, AFD receptive ending region. Asterisk, location of AWC cilium. Scale bar, 5 um. Dotted line in (D) outlines AMsh glia and in (E) outlines the animal.

Picture from Singhvi A et al. (2016) Cell "A Glial K/Cl Transporter Controls Neuronal Receptive Ending Shape by Chloride ...."

(A and B) Fluorescence images depicting expression of indicated transcriptional reporters. Box, cells of interest. Circles + line in (B) indicate distinct cells.

Picture from Singhvi A et al. (2008) Genetics "The T-box gene tbx-2, the homeobox gene egl-5 and the asymmetric cell division ...."

Figure 8. EGL-5 is expressed in the HSN/PHB precursor, while TBX-2 is not. (A-D) gmIs20[hlh-14::gfp]; muIs13[egl-5::lacZ] representative embryo around the 1.5-fold stage stained with anti-GFP (green) and anti-lacZ (red). (A) Schematic of embryo; (B) merge of anti-gfp and anti-lacZ; (C) anti-GFP; and (D) anti-lacZ. (A'-D') muIs13[egl-5::lacZ]; AW0095[tbx-2::gfp] representative embryo around the 1.5-fold stage stained with anti-GFP (green) and anti-lacZ (red). (A') Schematic of embryo; (B') merge of anti-gfp and anti-lacZ; (C') anti-GFP; and (D') anti-lacZ of a representative embryo.

Picture from Singhvi A et al. (2016) Cell "A Glial K/Cl Transporter Controls Neuronal Receptive Ending Shape by Chloride ...."

Figure 2. KCC-3 Localizes to Glial Membranes Surrounding AFD and Regulates KCl Homeostasis(A and B) Fluorescence images depicting expression of indicated transcriptional reporters. Box, cells of interest. Circles + line in (B) indicate distinct cells.(C) Histogram details as in Figures 1B and 1F. +, presence of kcc-3(ok228) allele. **p = 0.009.(D and E) Arrow, AFD receptive ending region. Asterisk, location of AWC cilium. Scale bar, 5 um. Dotted line in (D) outlines AMsh glia and in (E) outlines the animal. (F and G) Histogram details are as in Figures 1B and 1F. **p < 0.02. ns, not significant compared to age-matched kcc-3(ok228). Hatched bars, animals with disorganized AFD microvilli.

Picture from Singhvi A et al. (2016) Cell "A Glial K/Cl Transporter Controls Neuronal Receptive Ending Shape by Chloride ...."

Figure S4. GCY-8 Is Expressed at AFD Microvilli and Functions Downstream of KCl, Related to Figure 3(A) GCY-8 localization to AFD microvilli receptive endings. Arrow, AFD microvilli (zoomed in inset). Arrowhead, dendrite.(B) Histogram details as in Figure 1B.

Picture from Singhvi A et al. (2016) Cell "A Glial K/Cl Transporter Controls Neuronal Receptive Ending Shape by Chloride ...."

(C) Fluorescence image depicting expression of PDE-1 in AFD (dotted line).

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.