Picture from Zahreddine H et al. (2010) Curr Biol "CRT-1/calreticulin and the E3 ligase EEL-1/HUWE1 control hemidesmosome ...."

Figure 2. Expression Patterns and Fibrous Organelle Phenotypes of Selected evab Genes (A) Conserved domains of EEL-1 and CRT-1. Horizontal black bars indicate extent of deletion alleles ok1575 and ok948; arrow indicates position of GFP insertion. The following abbreviations are used: DUF, domain of unknown function; UBA, ubiquitin-associated domain; HECT, homologousto the E6-AP carboxyl terminus domain. (B) A transcriptional eel-1::gfp construct is expressed at low levels in most cells. (C-J) Immunostaining with NE8/4C6 (muscle; red) and VAB-10A (green) antibodies of vab-10A (e698) (C and H), crt-1(ok948) (D), eel-1(ok1575) (E), vab-10A(e698); crt-1(ok948) (F and I), and vab-10A(e698); eel-1(ok1575) embryos (G and J) at 1.7-fold (H-J) or 3-fold (C-G) stages. Antibodies from boxed areas in (Fa) and (Ga) are enlarged in (Fb) and (Fc) and (Gb) and (Gc), respectively. Arrows in (Fb) and (Gb) indicate areas with detached muscles but normal VAB10A; arrowheads in (Fb) and (Gb) indicate areas with abnormal VAB-10A; dotted lines in (Fc) and (Gc) indicate expected muscle positions; arrowheads in (I) and (J) indicate areas with detached muscles. Scale bar in (C) represents 10 um.

Picture from Norman KR et al. (2005) Cell "The Rho/Rac-family guanine nucleotide exchange factor VAV-1 regulates ...."

(I) VAV-1::GFP expression in the pharynx. GFP is observed in the epidermal cells (EC), pharyngeal muscle (PM), neurons (N), and gland-cell body (GB) and process (GP).(J) VAV-1::GFP expression in the spermatheca (s) and the sheath cells of the proximal gonad (pg). Arrowheads indicate sheath cells.(K) VAV-1::GFP expression in the posterior intestine (int) and rectal epithelial cells (rep). The location of the anus is indicated (a).

Picture from Norman KR et al. (2005) Cell "The Rho/Rac-family guanine nucleotide exchange factor VAV-1 regulates ...."

Figure 1. C. elegans vav-1 Encodes a Vav-Family GEF that Is Expressed in Pharynx, Gonad, and Intestine. (A) Amino acid sequence of VAV-1. Conserved tyrosines that are characteristic of Vav proteins (Y183, Y200, and Y217) are indicated by asterisks. A conserved leucine in the DH domain that is essential for GEF function is indicated by a diamond.(B) Domain structure of VAV-1. CH, calponin homology; AD, acidic; DH, Dbl homology; PH, plekstrin homology; C1, protein kinase C conserved region 1; SH2, Src homology 2; SH3, Src homology 3.(C) Image of a wild-type animal.(D-H) Transgenic worms expressing in muscle VAV-1::GFP (D); VAV-1::GFP(Y217F) (E); VAV-1(Y3XF) (F); VAV-1(N-delta) (G); VAV-1(N-delta);L260Q (H). For all animals, anterior is to the right.(I) VAV-1::GFP expression in the pharynx. GFP is observed in the epidermal cells (EC), pharyngeal muscle (PM), neurons (N), and gland-cell body (GB) and process (GP).(J) VAV-1::GFP expression in the spermatheca (s) and the sheath cells of the proximal gonad (pg). Arrowheads indicate sheath cells.(K) VAV-1::GFP expression in the posterior intestine (int) and rectal epithelial cells (rep). The location of the anus is indicated (a).