Picture from Shi A et al. (2010) Mol Biol Cell "EHBP-1 functions with RAB-10 during endocytic recycling in Caenorhabditis ...."

Figure 2. EHBP-1 is broadly expressed in C. elegans. Confocal images of EHBP-1-GFP transgene expression driven by the ehbp-1 promoter. Tissue-specific expression was observed as follows: (A) pharynx (arrows), nerve ring (arrowhead); (B) myofilament of body-wall muscle (arrows); (C) seam cells (arrows); (D) intestine (Top); (E) intestine (Middle); arrows indicate the basolateral intestinal membranes, and arrowheads indicate the apical intestinal membranes and basolateral cytoplasmic puncta; (F) spermatheca (arrowheads) and oviduct sheath cells plasma membrane (arrows). Scale bar, 10 um.

Picture from Shi A et al. (2010) Mol Biol Cell "EHBP-1 functions with RAB-10 during endocytic recycling in Caenorhabditis ...."

Figure 3. EHBP-1 colocalizes with RAB-10 and -8 on endosomes. All images are from deconvolved 3D image stacks acquired in intact living animals expressing GFP- and RFP-tagged proteins specifically in intestinal epithelial cells. (A-A'') Arrowheads, endosomes labeled by both EHBP-1-GFP and RFP-RAB-5. (B-B'') Arrowheads, endosomes labeled by both EHBP-1-GFP and RFP-RAB-10. (C-C'') Arrowheads, endosomes labeled by both EHBP-1-GFP and RFP-RAB-8. (D-D'') Arrowheads, endosomes labeled by both EHBP-1-GFP and RFP-RME-1. (E-E'') Arrowheads, EHBP-1-GFP-positive endosomes juxtaposed to RFP-RAB-11-labeled structures. (F-F'') EHBP-1-GFP does not colocalize with Golgi marker Mannosidase-RFP. In each image autofluorescent lysosome-like organelles can be seen in all three channels with the strongest signal in blue, whereas GFP appears only in the green channel and RFP only in the red channel. Signals observed in the green or red channels that do not overlap with signals in the blue channel are considered bone fide GFP or RFP signals, respectively. Scale bar, 10 um.

Picture from Shi A et al. (2007) Curr Biol "A novel requirement for C. elegans Alix/ALX-1 in RME-1-mediated membrane ...."

Figure 2. ALX-1 is Broadly Expressed in C. elegans.The expression of a GFP-ALX-1 transgene driven by the alx-1 promoter is indicated in (A-F). Scale bars represent 10 μm.(A) Intestine. Arrowheads indicate cytoplasmic puncta.(B) The pharynx is indicated by arrows, and the nerve ring is indicated by arrowheads.(C) The arrow indicates spermatheca, and the arrowhead indicates cytoplasmic puncta.(D) Coelomocytes, cytoplasmic puncta (arrowheads).(E) Nerve cord (arrowheads) and body-wall muscle (arrow).(F) Hypodermis, cytoplasmic puncta (arrowheads).

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.

Picture from Millonigg S et al. (2014) PLoS Genet "GLD-4-mediated translational activation regulates the size of the ...."

(A) GLD-4 expression is equal across the distal germ line. GLD-2 intensities increase from low-to-high in a distal-to-proximal manner. Extruded gonads of indicated genotype stained with DAPI, a-GLD-2, a-GLD-4, and a-GLH-2 as a positive tissue penetration control (not shown). Asterisk, distal tip; arrowhead, mitosis-to-meiosis boundary.

Picture from Xu XZS et al. (2003) Cell "A C. elegans sperm TRP protein required for sperm-egg interactions during ...."

Figure 3. The TRP-3 Protein Is Highly Enriched in Sperm. (A-H) An affinity-purified antibody against TRP-3 specifically stains sperm in wild-type hermaphrodites. Scale bar = 10 μm.(A) An immunofluorescence image of anti-TRP3 staining of a wild-type hermaphrodite.(B) The same animal in (A) stained with DAPI.(C) A superimposed image of (A) and (B).(D) A DIC image of the same animal in (A-C).(E-H) The TRP-3 antibody is specific.(E) The TRP-3 antibody does not detect a signal in trp-3(sy693) mutants.(F) A DAPI image of the same animal shown in (E).(G) A superimposed image of (E) and (F).(H) A DIC image of the same animal shown in (E-G).