Picture from Gaiser AM et al. (2011) PLoS One "Downregulation of the Hsp90 system causes defects in muscle cells of ...."

Figure 2. daf-21 is ubiquitously expressed in Caenorhabditis elegans. A-C. Nematodes of the BC10293 strain, which contains a transcriptional fusion of GFP to the daf-21 promoter are shown. Fluorescence can be detected in the excretory canal cell (long arrow), in the pharyngeal muscle cells (sharp triangle) and the cells of the first intestinal ring (asterisk). Further individual muscle cells (blunt triangle) and the vulval muscles (short arrow) show fluorescence (A). Detailed views of the same strain highlight the excretory canal cell (long arrow), an individual muscle cell (blunt triangle) (B), the pharyngeal muscle cells (sharp triangle) and the cells of the first intestinal ring (asterisk) (C). D-E. Newly generated transgenic nematodes, carrying a daf-21 promoter sequence fused to YFP. Fluorescence is detected in the excretory canal cell (long arrow) and body wall muscle cells (wide triangles) (D). Fluorescence is also pronounced in some head neurons (E). The scale bar represents 40 mm in all subpanels.

Picture from Asano A et al. (2003) Curr Biol "Claudins in Caenorhabditis elegans: their distribution and barrier function in ...."

CLC-2 in C. elegans (A) A reporter, which fuses GFP to the predicted last amino acid of the CLC-2 protein (CLC-2-GFP) and is driven by 2.0 kb of the genomic sequence upstream of the endogenous CLC-2 gene, was constructed. In adult stable transformants expressing CLC-2-GFP, intense GFP signals were detected in hypodermal seam cells (arrows in [a] and [b]), which fuse to form a single elongated syncytium, although the GFP signal did not appear to be concentrated at cell-cell borders. When adult wild-type worms were whole-mount stained with anti-CLC-2 pAb, the seam cell syncytium appeared to be outlined to give two parallel lines, although these lines were thick and not very sharp (arrow in [c]). The scale bars represent 10 um.

Picture from Fu Y et al. (2009) Sci Signal "Neuronal and intestinal protein kinase d isoforms mediate Na+ (salt ...."

Fig. 4. DKF-2B is expressed in 20 head and tail neurons. Animals expressing a dkf-2B::DKF-2B-GFP transgene were analyzed by fluorescence microscopy to determine DKF-2B localization. Full-length DKF-2B-GFP is found in a limited number of head and tail neurons in L2 larvae (A1) and adult (B1) animals. (A2) and (B2) are bright-field images of (A1) and (B1), respectively. (C) Head neurons expressing DKF-2B-GFP. (D) Tail neurons expressing DKF-2B-GFP. Names of neurons are indicated. Thirteen neurons that express DKF-2B-GFP are in sharp focus in (C) and (D). Diagrams in (E) (head) and (F) (tail) depict locations of neurons (colored cell bodies) expressing DKF-2B-GFP in the context of the organization of the nervous system and anatomy of C. elegans. Cells that express DKF- 2A-GFP in vivo were identified previously (20, 21).

Picture from Chen D et al. (2010) Science "Retromer is required for apoptotic cell clearance by phagocytic receptor ...."

Figure 3. snx-1(tm847) causes defective recycling of internalized CED-1. (A) Time-lapse chasing of CED-1::GFP (arrows) and LMP-1::mCherry (sharp arrowheads) on phagosomes in N2 and snx-1(tm847) animals. Clustering of CED-1::GFP on the phagocytic cup was set as 0 min. Blunt arrowheads indicate membranes of engulfing cells. Bars, 5 um. (B) Representative images (top) and quantification (bottom) of CED-1::GFP and LMP-1::mCherry colocalization on cell corpses at a late stage of degradation in N2 and snx-1(tm847) embryos. At least 100 cell corpses in each strain were examined. Arrows indicate cell corpses. DIC, differential interference contrast. Bars, 5 um. (C) Localization of CED-1::GFP and LMP-1::mCherry in hypodermal cells in N2 and snx-1(tm847) embryos. Bars, 10 um.

Picture from Barriere A et al. (2012) PLoS Genet "Coevolution within and between regulatory loci can preserve promoter function ...."

Figure 1. The expression pattern of unc-47 is conserved despite divergent regulation. (A) Both C. elegans and C. briggsae promoters fused upstream of GFP in their endogenous trans-regulatory environments drive expression in all 26 GABAergic neurons (green). However, the C. briggsae promoter placed in the C. elegans trans environment additionally drives expression in SDQR and SDQL (blue). (B) Those cells were identified as SDQR/L based on their position and their characteristic projections. (C) For each combination of promoter and trans-regulatory environment, expression in SDQR and SDQL is presented. C. elegans is represented by straight lines, C. briggsae by wavy lines. Frequency of expression is represented by the width, and intensity of expression relative to D-type neurons by the height of black boxes. Number of individuals expressing and total number of individuals scored is indicated underneath. Measurements for independent strains are given in Figure S1. Differences in frequency of expression in SDQR are significant for all comparisons: C. elegans and C. briggsae promoters in C. elegans trans environment (p = 8.1610212), C. elegans and C. briggsae promoters in C. briggsae trans environment (p = 1.561028), C. elegans promoter in C. elegans and C. briggsae trans environments (p = 4.8610211), C. briggsae promoter in C. elegans and C. briggsae trans environments (p = 2.4610214). (D) Interpretation of the results presented in panel C. Both C. elegans and C. briggsae promoters in their endogenous trans environments drive low levels of expression in SDQR and SDQL, while disruption of the endogenous interactions either drives high levels of expression (C. briggsae promoter in C. elegans) or abolishes expression (C. elegans promoter in C. briggsae).

Picture from Beaster-Jones L et al. (2004) J Mol Biol "DNA binding and in vivo function of C.elegans PEB-1 require a conserved FLYWCH ...."

Figure 2. Cb-PEB-1 and Ce-PEB-1 are expressed in similar pattern. Fluorescence micrographs of 1.5-fold stage C. briggsae (A) and C. elegans embryos (B) stained with C. elegans PEB-1 antibodies (anterior is left). Staining is most abundant in pharyngeal nuclei in both C. briggsae and C. elegans (brackets). Fainter staining is visible in hypodermal cells and the hindgut in C. elegans and C. briggsae, although all staining was generally weaker in C. briggsae.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(C, C') Pgrl-2::GFP is expressed in the excretory duct and pore cells.

Picture from Barkoulas M et al. (2016) PLoS Genet "Evolution of New cis-Regulatory Motifs Required for Cell-Specific Gene ...."

(B-E) smFISH using a lin-3 probe in C. elegans N2 (data from [2]) (B), C. briggsae AF16 (C), C. angaria RGD1 (D) and O. tipulae CEW1 (E). Red arrow marks the position of the anchor cell.

Picture from Hobert O et al. (1999) J Cell Biol "A conserved LIM protein that affects muscular adherens junction integrity and ...."

(A-C): UNC-97::GFP localization in live worms at embryonic stage 300 min (A), 400 min (comma stage) (B), and 500 min (threefold pretzel stage) (C). (A'-C') Corresponding DIC micrographs. White arrows in C, a muscle nucleus and dense bodies, respectively. Note UNC-97::GFP localization to the periphery of nuclei in A-C.

Picture from Vazquez-Manrique RP et al. (2007) Genomics "The frataxin-encoding operon of Caenorhabditis elegans shows complex ...."

(a-c) Expression of F59G1.1::gfp in pharynx and gut (a), anal cells (b), and spermatheca (sph) (c).