Figure 4. Localization of SAX-3::GFP in PDE neuron. (A) Quantitation of the abnormal SAX-3::GFP distribution in some mutants. Any SAX-3::GFP distribution that is different from (B) is regarded as abnormal. Asterisks denote statistically significant difference between the wild-type worms and the mutants. P,0.01, P,0.05. (B) In wild-type animals, SAX-3::GFP distributed mainly on cell membrane and axons, and was also evenly expressed in the cytoplasm. Scale bar: 5 um. (C)
unc-51(
e1189) mutants showed altered SAX-3::GFP distribution in cytoplasm (with aggregates in cell body). Arrow: SAX-3::GFP aggregation. Scale bar: 5 mm. (D) SAX-3::GFP aggregates in the PDE cell body of the
unc-51 RNAi worm. Arrow: SAX-3::GFP aggregation. Scale bar: 5 mm. P,0.001. ngEx31(Pdat-1::SAX-3::GFP) or ngIs15(Pdat-1::SAX-3::GFP) was crossed to the mutants to examine the membrane localization of SAX-3::GFP. RNAi experiment was performed on
rrf-3(
pk1426);ngIs15(Pdat-1::SAX-3::GFP) worms. Error bars represent standard errors.