Figure 1. JU1171, LKC34, and N2 males were less likely to mate successfully and produced significantly fewer viable cross progeny at 27°C: Males were either raised at 20°C (blue bars) or upshifted to 27°C at L1 stage (red bars) and crossed with either strain matched or
unc-24(
e138) (Unc) hermaphrodites. (A-C) The percentage of males that mated successfully and produced viable cross progeny (% Successful males) was assessed. Significantly fewer males of all three wild type strains mated successfully to produce viable cross progeny at 27°C versus 20°C (Fisher's Exact Test, *P < 0.05). (D-F) The number of viable cross progeny produced by successful males was assessed (dead eggs and unfertilized oocytes were not counted). Males of all three wild type strains produced significantly fewer viable cross progeny at 27°C versus 20°C (Two-way ANOVA with Tukey's correction, *P < 0.05). Error bars represent ± 1 SEM.