Picture from Kelley CA et al. (2018) Mol Biol Cell "The myosin light-chain kinase MLCK-1 relocalizes during Caenorhabditis ...."

FIGURE 3: MLCK-1 is expressed in the C. elegans spermatheca and localizes to apical cell boundaries and basal actinbundles. (A) Confocal images of animals from L4 to young adult expressing MLCK-1 tagged with mKate2 at theendogenous locus. MLCK-1 expression is seen in the spermatheca throughout development. (B) Magnified maximumintensity projections of the spermathecae from the animals in A indicated with dashed boxes. The Fire color scale isused to highlight changes in fluorescence intensity. MLCK-1 expression increases from L4 to young adulthood and isprimarily localized apically. In mature adults, a subpopulation of MLCK-1 is observed at the basal surface. (C) Confocalimages of a spermatheca after the first ovulation from an animal coexpressing MLCK-1::mKate2 (red) and AJM-1::GFP(blue), which localizes near the apical surface of the spermathecal cells. Maximum-intensity projections show theproximity of MLCK-1 and AJM-1, with MLCK-1 immediately apical to AJM-1. The insert is a magnified cross-section fromthe area indicated with a dashed line, with basal at the bottom. (D) Confocal images of a WT excised spermatheca afterthe first ovulation expressing MLCK-1::mKate2 and stained with phalloidin to visualize F-actin. Maximum-intensityprojections show actin bundles and MLCK-1 localization. The cross-section shows that prominent actin bundles are basaland a small population of MLCK-1 colocalizes with these bundles. Inserts are a magnified cross-section of the areaindicated with a dashed line. Basal is at the bottom. Note that the brightness in the insert in D is enhanced to facilitatesimultaneous visualization of thick basal actin bundles and the thin apical actin network. (E) Quantification of a line scandrawn across the apical cell surface in the area indicated by the insert in C. The fluorescence peak for AJM-1::GFP is justbasal to MLCK-1::mKate2 peak. (F) Quantification of a line scan drawn across the area indicated in the insert in (D) fromthe basal to the apical cell surface. The majority of the MLCK-1::mKate2 signal is apical and a small peak aligns with thebasal fluorescence peak for F-actin. Scale bar, 100 um in A, 20 um in B-D, and 5 um in the inserts in C and D. Ap., apical;Bs., basal.