Picture from Granato M et al. (1994) Development "Genesis of an organ: molecular analysis of the pha-1 gene."

Figure 4. Northern analysis of the pha-1 transcript. A single transcript is detected at different stages of development using the 6.6 kb genomic fragment that rescues the pha-1 phenotype as a probe on northern blots. Low level of pha-1 transcript is detectable during the early stages of embryonic development (0-2 hours). At 3-5 hours of development pha-1 expression is increased, consistent with the temperature-sensitive period of pha-1 (e2123ts) (Schnabel and Schnabel, 1990). The mRNA is also expressed at lower levels in larval stages. No genetic requirement of pha-1 during the larval stages was observed. Below: 28S rRNA stained with methylene blue as a control for loading equal amounts of RNA.

Picture from Ginzburg VE et al. (2002) Development "Semaphorin 1a and semaphorin 1b are required for correct epidermal cell ...."

smp-2 expression patterns visualized with gfp transcriptional reporters during embryogenesis. DIC (left) and GFP (right) images of the same embryo. (G,H) Initial embryonic expression of smp-2::gfp is observed at the twofold stage in pharyngeal (arrows) and intestinal (arrowheads) cells. These cells were identified using SIMI BioCell CD ROMTM (Schnabel et al., 1997).

Picture from Susoy, Vladislav et al. (2019) MicroPubl Biol

Figure 1. Psto3::wrmScarlet reporter is expressed in R4BL/R and R8BL/R ray neurons.

Picture from Etchberger JF et al. (2007) Genes Dev "The molecular signature and cis-regulatory architecture of a C. elegans ...."

The srd-33 7TMR reporter gene fusion also shows a restricted expression pattern (ASEL/R, AWBL/R, ASHL/R, AVKL/R, PHAL/R, PHBL/R, and weakly in another unidentified tail neuron pair, possibly PHCL/R). Each data set of micrographs shows expression of the reporter construct under investigation in a high-magnification (400x) image, the overlap with a red fluorescent marker for ASEL/ASER (otIs151 transgene; 'ASE-RFP'), and a low-magnification (160x) overview of expression of the reporter construct under investigation throughout the whole worm. In the low-magnification images, the asterisk (*) indicates expression of the gut-specific injection marker elt-2::gfp.

Picture from Lin L et al. (2013) J Cell Biol "The scaffold protein EPG-7 links cargo-receptor complexes with the autophagic ...."

At larval stages, W07G4.5::GFP forms aggregates in intestinal cells (Q and R).

Picture from Yan D et al. (2009) Cell "The DLK-1 kinase promotes mRNA stability and local translation in C. elegans ...."

Figure S7. Colocalization analysis of MAK-2 and CEBP-1 with presynapticmarkersAll images are of synaptic regions of the dorsal processes of GABAergic motorneurons. For each panel, pixel values obtained from line scans of a confocalprojection (on left) are plotted on the right. Pearson's correlation coefficient r and the probability were calculated. (A) mCherry::MAK-2 (juEx1503) partly colocalizes with SNB-1::GFP (juIs1). r = 0.69, P < 0.0001. (B) In rpm-1(lf) mutants mCherry::MAK-2 and SNB-1::GFP are aberrantly distributed but remain colocalized. (C) mCherry::MAK-2 does not colocalize with RPM-1::GFP (juIs77). r = -0.14, P = 0.054. (D) mCherry::CEBP-1 (juEx1735) colocalizes with SNB1::GFP. r = 0.88, P < 0.0001.(E) mCherry::CEBP-1 is excluded from areas of RPM-1::GFP. r = -0.67, P < 0.001. (F) mCherry::MAK-2 and CEBP-1::GFP (juEx2157) partly colocalize; r = 0.48, P < 0.0001. Scale, 10 um.

Picture from Mariner BL et al. (2023) MicroPubl Biol "Easy C. elegans Worm Lifespan Plotting and Statistics with ...."

Figure 1. Example plot generated from WLSplot workflow:Visualization of WLS_autoplot() features when plotting worm lifespan data with the WLSplot R package.

Picture from Etchberger JF et al. (2007) Genes Dev "The molecular signature and cis-regulatory architecture of a C. elegans ...."

Figure 2. Expression patterns of genes from the SAGE library as assessed by gfp reporter gene technology. Only a selected and representative number of examples are shown in this figure. All analyzed reporter strains, including those shown here, are shown in Supplementary Figures 1-3. Green boxes indicate gfp coding sequences (rfp in the case of dop-3 and yfp in the case of hlh-10). If extrachromosomal arrays were generated, multiple lines were analyzed for each reporter construct, as indicated below each panel of micrographs. Each data set of micrographs shows expression of the reporter construct under investigation in a high-magnification (400x) image, the overlap with a red fluorescent marker for ASEL/ASER (otIs151 transgene; 'ASE-RFP'), and a low-magnification (160x) overview of expression of the reporter construct under investigation throughout the whole worm. In the low-magnification images, the asterisk (*) indicates expression of the gut-specific injection marker elt-2::gfp. (A) Expression patterns of two selected TFs from the SAGE data set. (B) Expression patterns of two selected peptidergic signaling proteins from the SAGE data set. (C) Expression patterns of two selected putative chemoreceptor proteins of the 7TMR family from the SAGE data set. The srg-30 7TMR-type chemoreceptor shows the most restricted expression pattern with strong expression in ASEL/R and weak expression in the chemosensory neurons ASIL/R and ADLL/R. Additional expression can be observed in a pharyngeal neuron. The srd-33 7TMR reporter gene fusion also shows a restricted expression pattern (ASEL/R, AWBL/R, ASHL/R, AVKL/R, PHAL/R, PHBL/R, and weakly in another unidentified tail neuron pair, possibly PHCL/R). The bottom panels in C demonstrate that 7TMR-type chemoreceptors localize to the dendritic ending of the ASE neurons. In order to boost expression in the ASE neurons, a 188-bp ASEL-specific regulatory element from the gcy-7 gene was appended to each locus (indicated as a black box). One transgenic line each was analyzed in detail. Yellow arrows point to the dendritic endings.

Picture from Ginzburg VE et al. (2002) Development "Semaphorin 1a and semaphorin 1b are required for correct epidermal cell ...."

Figure 8. smp-1 and smp-2 expression patterns visualized with gfp transcriptional reporters during embryogenesis. DIC (left) and GFP (right) images of the same embryo. (A,B) The expression pattern of smp-1::gfp can be initially observed at approximately 150 minutes in four cells of the E lineage and later at about 250 minutes of development spread to eight cell of the same lineage. (C,D) At the time of ventral enclosure, the leading edge epidermal cells and the muscle lineage start to express smp-1::gfp. (E,F) In the comma stage, muscle precursors and other cells in the head express smp-1::gfp. (G,H) Initial embryonic expression of smp-2::gfp is observed at the twofold stage in pharyngeal (arrows) and intestinal (arrowheads) cells. These cells were identified using SIMI BioCell CD ROMTM (Schnabel et al., 1997).

Picture from Lu Q et al. (2011) Dev Cell "The WD40 repeat PtdIns(3)P-binding protein EPG-6 regulates progression of ...."

Q-T. Expression of gfp::epg-6 in the head region (Q), body wall muscles (R), the tail region (S) and the ventral cord (T) at larval stages.