Picture from Sumakovic M et al. (2009) J Cell Biol "UNC-108/RAB-2 and its effector RIC-19 are involved in dense core vesicle ...."

Figure S1. rab-2 shows ubiquitous expression with high expression levels in the nervous system. (A-C) GFP was expressed under the rab-2 promoter. rab-2 is expressed in the muscle (M) but shows strong expression levels in the nervous system. BWM, body wall muscle; HN, head neurons; NC, nerve cord; TG, tail ganglia; VM, vulva muscle. (D) Expression of rab-2 in vulval cells (VC) and intestinal epithelium (IE). Bars: (A) 30 um; (B-D) 20 um.

Picture from Gobel V et al. (2004) Dev Cell "Lumen morphogenesis in C. elegans requires the membrane-cytoskeleton linker ...."

Figure 1. ERM-1 Is Enriched at Luminal Surfaces. Here and elsewhere, animals are viewed from the side: anterior left, posterior right, dorsal top, ventral bottom. Gray boxes in schematics (iA, iiA, and iiiA) indicate areas magnified in images beneath. (iC and iiB) Nomarski images; (iD and iF) confocal sections; (iE, iiC, iiiB, and iiiC) confocal projections.i. Intestine. (A) ERM-1 localizes to intestinal lumen (green). (B) Wild-type intestine with 20 bilaterally symmetrical cells in 9 rings (4 cells for first ring; blue), intestinal lumen (green), AJ (red); tube is rotated along longitudinal axis. (C-F) Anterior intestinal tube; apical (small arrowheads) and basal (large arrowheads) sides of tube indicated. ERM-1-GFP (D and E) is at the lumen, apical to but distinct from α-AJM-1 (yellow in [E] from integration of sections, not overlap). ERM-1-GFP also seen in excretory canal in (E) (arrow). NFM-1-lacZ (F) is localized basolaterally.ii. Excretory canals. (A) ERM-1 localizes to excretory canals (red; canals shown separated for clarity). (B and C) Anterior excretory canals: ERM-1-GFP outlines canals and also the intestinal (arrow), but not pharyngeal (arrowhead) lumen.iii. Gonad. (A) ERM-1 localizes to gonad lumina: spermathecae, uterus, vulva (blue). (B) Integration of sections through uterine-vulval connection (ventral view); vulva opens to right: ERM-1-GFP outlines eggs in uterus and vulval opening (arrowhead). (C) Vulva (large arrowhead) at higher magnification (ventrolateral view; vulva opens to right): ERM-1-GFP is separate from complex junctional α-AJM-1 pattern (yellow from integration of sections, not overlap); intestinal lumen at left (small arrowhead).

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This pattern is first expressed as a few nuclei in 3-fold embryos. A few nuclei also stain in young larvae. In adults expression is evident posteriorly to the vulva. This expression is reproducible but seems to be on the threshhold of observability as sometimes expression is not seen (due to less efficient staining?) It appears to stain the A and B epithelial cells which connect the uterus to the hypodermal syncytium, ie in the hypodermal ridge posterior to the vulva. We have seen one example (due to the low threshhold?) where the posterior third of the anterior component of the A and B epithelial cells, as well as the posterior component stain. This does seem to follow with the possibility of some concentration gradient evident in the gene product produced. There is occasionally a second component. This is seen in the larval stages and is anterior, and more frequently posterior, intestinal y nuclei. In adults some diffuse intestinal staining is also seen.

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This pattern is first expressed as a few nuclei in 3-fold embryos. A few nuclei also stain in young larvae. In adults expression is evident posteriorly to the vulva. This expression is reproducible but seems to be on the threshhold of observability as sometimes expression is not seen (due to less efficient staining?) It appears to stain the A and B epithelial cells which connect the uterus to the hypodermal syncytium, ie in the hypodermal ridge posterior to the vulva. We have seen one example (due to the low threshhold?) where the posterior third of the anterior component of the A and B epithelial cells, as well as the posterior component stain. This does seem to follow with the possibility of some concentration gradient evident in the gene product produced. There is occasionally a second component. This is seen in the larval stages and is anterior, and more frequently posterior, intestinal y nuclei. In adults some diffuse intestinal staining is also seen.

WBPicture0000006580

This pattern is first expressed as a few nuclei in 3-fold embryos. A few nuclei also stain in young larvae. In adults expression is evident posteriorly to the vulva. This expression is reproducible but seems to be on the threshhold of observability as sometimes expression is not seen (due to less efficient staining?) It appears to stain the A and B epithelial cells which connect the uterus to the hypodermal syncytium, ie in the hypodermal ridge posterior to the vulva. We have seen one example (due to the low threshhold?) where the posterior third of the anterior component of the A and B epithelial cells, as well as the posterior component stain. This does seem to follow with the possibility of some concentration gradient evident in the gene product produced. There is occasionally a second component. This is seen in the larval stages and is anterior, and more frequently posterior, intestinal y nuclei. In adults some diffuse intestinal staining is also seen.

WBPicture0000006577

This pattern is first expressed as a few nuclei in 3-fold embryos. A few nuclei also stain in young larvae. In adults expression is evident posteriorly to the vulva. This expression is reproducible but seems to be on the threshhold of observability as sometimes expression is not seen (due to less efficient staining?) It appears to stain the A and B epithelial cells which connect the uterus to the hypodermal syncytium, ie in the hypodermal ridge posterior to the vulva. We have seen one example (due to the low threshhold?) where the posterior third of the anterior component of the A and B epithelial cells, as well as the posterior component stain. This does seem to follow with the possibility of some concentration gradient evident in the gene product produced. There is occasionally a second component. This is seen in the larval stages and is anterior, and more frequently posterior, intestinal y nuclei. In adults some diffuse intestinal staining is also seen.

WBPicture0000005754

This neuronal pattern gives expression throughout all life stages of C. elegans. Staining is first seen in precomma stage embryos. In 3-fold embryos expression appears to be localised in nuclei around the pharynx and tail. In young larvae there is extensive staining in all of the head ganglia and the anal ganglion. The ventral nerve cord and its cell bodies also show strong expression. As the worm ages the expression in the head ganglia is reduced to a number of nuclei in the ventral (ie AIML/R?) and lateral ganglion, although expression in the nerve ring and the ventral nerve cord is still observed. There appears to be some mosaicism in the expression as some larvae show stronger staining in the ventral nerve cord and more nuclei stain in the head ganglia, whereas in other larvae head expression is reduced and only the cell bodies of the ventral nerve cord stain. Diffuse expression is sometimes observed in the metacorpus and terminal bulb of the pharynx in larvae and adults.

WBPicture0000005756

This neuronal pattern gives expression throughout all life stages of C. elegans. Staining is first seen in precomma stage embryos. In 3-fold embryos expression appears to be localised in nuclei around the pharynx and tail. In young larvae there is extensive staining in all of the head ganglia and the anal ganglion. The ventral nerve cord and its cell bodies also show strong expression. As the worm ages the expression in the head ganglia is reduced to a number of nuclei in the ventral (ie AIML/R?) and lateral ganglion, although expression in the nerve ring and the ventral nerve cord is still observed. There appears to be some mosaicism in the expression as some larvae show stronger staining in the ventral nerve cord and more nuclei stain in the head ganglia, whereas in other larvae head expression is reduced and only the cell bodies of the ventral nerve cord stain. Diffuse expression is sometimes observed in the metacorpus and terminal bulb of the pharynx in larvae and adults.

WBPicture0000005757

This neuronal pattern gives expression throughout all life stages of C. elegans. Staining is first seen in precomma stage embryos. In 3-fold embryos expression appears to be localised in nuclei around the pharynx and tail. In young larvae there is extensive staining in all of the head ganglia and the anal ganglion. The ventral nerve cord and its cell bodies also show strong expression. As the worm ages the expression in the head ganglia is reduced to a number of nuclei in the ventral (ie AIML/R?) and lateral ganglion, although expression in the nerve ring and the ventral nerve cord is still observed. There appears to be some mosaicism in the expression as some larvae show stronger staining in the ventral nerve cord and more nuclei stain in the head ganglia, whereas in other larvae head expression is reduced and only the cell bodies of the ventral nerve cord stain. Diffuse expression is sometimes observed in the metacorpus and terminal bulb of the pharynx in larvae and adults.

WBPicture0000005758

This neuronal pattern gives expression throughout all life stages of C. elegans. Staining is first seen in precomma stage embryos. In 3-fold embryos expression appears to be localised in nuclei around the pharynx and tail. In young larvae there is extensive staining in all of the head ganglia and the anal ganglion. The ventral nerve cord and its cell bodies also show strong expression. As the worm ages the expression in the head ganglia is reduced to a number of nuclei in the ventral (ie AIML/R?) and lateral ganglion, although expression in the nerve ring and the ventral nerve cord is still observed. There appears to be some mosaicism in the expression as some larvae show stronger staining in the ventral nerve cord and more nuclei stain in the head ganglia, whereas in other larvae head expression is reduced and only the cell bodies of the ventral nerve cord stain. Diffuse expression is sometimes observed in the metacorpus and terminal bulb of the pharynx in larvae and adults.