Figure 5. Subcellular localization of CUP-2. (A) Confocal images of
pmyo-3::ssGFP or
cup-2(
ar506);
pmyo-3::ssGFP adult hermaphrodites (left panels) and individual coelomocytes (right panels) expressing a CUP-2::GFP translational fusion from a coelomocyte-specific promoter. Worms were grown at 20C. (B) Quantification of the sizes of the GFP-filled vesicles in coelomocytes of strains shown in A. 1 pixel=0.001
um2. (C) Average intensity per pixel of GFP in the nuclei of coelomocytes of strains of the indicated genotypes expressing HSP-4::GFP. Worms were grown at 20C. (D) Confocal images of coelomocytes co-expressing CUP-2::GFP and the smooth ER (sER) marker cytochrome
b5 or CUP-2::GFP and the rough ER (rER) marker TRAM or CUP-2::GFP and the early endosome marker RAB-5, fused to mCherry. Arrows indicate structures that only contain CUP-2::GFP in the ER localization images and endosomes that contain both CUP-2 and RAB-5 in the bottom panels. Worms were grown at 20C.