Picture from Hayashi M et al. (2007) PLoS Genet "C. elegans germ cells switch between distinct modes of double-strand break ...."

Figure 2. Altered Profile of RAD-51 Foci in rad-50 Mutant Germ Lines (A) Images show a portion of the germ lines of wild type and rad-50 mutant hermaphrodites, extending from the premeiotic region in which nuclei have not yet entered meiotic prophase (left) through the region of meiotic prophase entry (transition zone) and into the pachytene region (in which chromosomes are fully paired and synapsed; right). Chromosomes are stained with DAPI and RAD-51 antibody. In the wild-type germ line, RAD-51 foci are very infrequent in premeiotic and transition zone nuclei, but are abundant in pachytene nuclei, with multiple foci in the majority of nuclei. In the rad-50 mutant germ line, the number of foci in premeiotic and transition zone nuclei is elevated, while the number of foci in pachytene nuclei is severely reduced. Scale bar = 5 um. (B) Quantitative time course analysis of RAD-51 foci. Stacked bar graph depicting quantitation of RAD-51 foci in premeiotic nuclei (pm), transition zone nuclei (tz), early-mid pachytene nuclei (e/m pt), and late pachytene nuclei (l pt) in gonads of the indicated genotypes. Different colored segments represent the percentage of nuclei scored that had the numbers of RAD-51 foci indicated by the color code at the right of the graph. (C) High magnification view of pachytene nuclei from wild type and spo-11; rad-50 germ lines. Whereas the RAD-51 foci in the wild-type pachytene nuclei are more numerous, the SPO-11-independent RAD-51 foci in the spo-11; rad-50 nuclei are larger/brighter. Scale bar = 5 um.

Picture from Kagawa H et al. (1995) J Mol Biol "Genome structure, mapping and expression of the tropomyosin gene tmy-1 of ...."

Figure 5. Tissue-specific expressions of the tmy-1 gene. A fusion gene containing the tmy-1 gene and lacZ was used to study cellular expression. C. elegans preparations were histochemically stained for b-galactosidase expression using X- Gal as a substrate (A to F) and for tropomyosin isoforms with affinity-purified antibodies (G & H). A, Staining of the body wall muscles, pTMIZ2328, L3; B, staining of the male tail muscles, pTMIZ2328; C, staining of body-wall muscles, pT- MIZ2328, adult; D, staining of the pharyngeal muscles, pTMIII3256, adult; E, staining of L1 worm, pTMIZ818; F, staining of the pharyngeal muscles, pTMIIIZ3256-Bg, L1 worm; G, staining of the body-wall muscles with affinity-purified anti- CeTMI; H, staining of the pharyngeal muscles with affinity-purified anti-CeTMIII. Scale bars represent 50 um. These results indicate that the 5' end promoter and the internal promoter in the third intron control the expression of the body wall and the pharyngeal muscles, respectively.