Picture from Starich TA et al. (1993) Genetics "Molecular and genetic analysis of unc-7, a Caenorhabditis elegans gene required ...."

FIGURE 5. Transcript levels of unc-7 vary over the course of development. RNA was isolated from eggs (lane 1). the four larval stages LI (lane 2). L2 (lane 3).L3 (lane 4). and L4 (lane 5),and young adults(lane6). A northern blot containing total RNA, 30pg per lane, was hybridized to unc-7 coding sequences (Ao) r an rRNA probe (B, showing 18s signal). Poly(A)-enriched RNA, 10 ug per lane (C), was hybridized to the same unc-7 probe as in A.

Picture from Jaramillo-Lambert A et al. (2010) Curr Biol "Meiotic errors activate checkpoints that improve gamete quality without ...."

Figure 5. The Proapoptotic Machinery Is Expressed in the Male Germline during Meiotic Prophase, but CED-3 Caspase Is Not Activated. (A) Pathway of germ cell apoptosis in C. elegans [53].(B) CED-9 inactivation does not induce apoptosis in the male germline. Number of apoptotic nuclei per gonad arm measured by AO staining 24 hr post L4 stage (and 24 hr post IR) is indicated. The number of germlines scored for each genotype is in parenthesis. The data shown are means ± SEM.(C) CED-4 and SIR-2.1 are expressed in both hermaphrodite and male germlines, but no SIR-2.1 translocation occurs in male germ cells. Images show immunolocalization of CED-4 (green) and SIR-2.1 (red) counterstained with DAPI (blue) in pachytene nuclei from wild-type and zim-1(tm1813) hermaphrodite and male germlines. Arrowheads point to nuclei with more intense CED-4 staining. Scale bars represent 5 μm.(D) CED-3 is expressed in hermaphrodite and male germlines, but levels do not increase under checkpoint-activating conditions in the male germline. Images show CED-3 immunolocalization (green) in pachytene nuclei stained with DAPI (blue) of adult worms (24 hr post L4). Arrowheads indicate apoptotic nuclei that lack CED-3 staining presumably because they have proceeded to a later stage. Scale bars represent 5 um.(E) Males do not activate CED-3 caspase. Caspase activity was monitored by fluorescence microscopy following microinjection of SR-FLICA inhibitor in hermaphrodite and male germlines of WT, zim-1(tm1813), and zim-1(tm1813);ced-3(RNAi) worms. Arrowheads indicate some of the caspase-positive cells. Scale bars represent 10 μm.See also Figure S3.