Picture from Saito TT et al. (2009) PLoS Genet "Caenorhabditis elegans HIM-18/SLX-4 interacts with SLX-1 and XPF-1 and ...."

Immunolocalization of HIM-18 in germline nuclei of wild type hermaphrodites. Low magnification images show nuclei progressing from the premeiotic tip through all the stages of meiotic prophase, in a left to right orientation. Arrowheads indicate the beginning of transition zone and entrance into meiosis. A single focal plane from a representative nucleus of each stage is shown at a higher magnification. Detection thresholds for anti-HIM-18 signals in transition zone and mid-pachytene panels were lower than for other panels to permit imaging of the lower levels of chromosome-associated HIM-18 observed in these stages. Bars, 20 um for whole gonad, 1 um in insets.

Picture from Saito TT et al. (2009) PLoS Genet "Caenorhabditis elegans HIM-18/SLX-4 interacts with SLX-1 and XPF-1 and ...."

Figure 1. HIM-18 is a conserved protein that localizes in both mitotic and late meiotic prophase germline nuclei. (A) Schematic representation of the predicted HIM-18 protein structure. The region deleted in the tm2181 mutant allele is indicated (codons 161 through 277). The various conserved domains are indicated by the following abbreviations: ZF, zinc finger; CC, coiled coil; BTB, Bric-a-brac, Tramtrack, Broad-complex; SAP, SAF-A/B, Acinus and PIAS; LZ, leucine zipper. The black bar indicates the N-terminal region used for antibody production. (B) Schematic representation of Caenorhabditis elegans HIM-18 and related predicted proteins in Homo sapiens, Mus musculus, Drosophila melanogaster, Saccharomyces cerevisiae, and Schizosaccharomyces pombe. Protein names and accession numbers are indicated. Percent identity and similarity (I and S, respectively) are indicated in parentheses. The brown horizontal bar indicates the region of homology in Dm-MUS312 shared with Sc-Slx4. (C) Immunolocalization of HIM-18 in germline nuclei of wild type hermaphrodites. Low magnification images show nuclei progressing from the premeiotic tip through all the stages of meiotic prophase, in a left to right orientation. Arrowheads indicate the beginning of transition zone and entrance into meiosis. A single focal plane from a representative nucleus of each stage is shown at a higher magnification. Detection thresholds for anti-HIM-18 signals in transition zone and mid-pachytene panels were lower than for other panels to permit imaging of the lower levels of chromosome-associated HIM-18 observed in these stages. Bars, 20 um for whole gonad, 1 um in insets.

Picture from Saito TT et al. (2024) MicroPubl Biol "Caenorhabditis elegans brc-1 mutation increases the number of COSA-1 ...."

Figure 1. Deletion of BRC-1 increases the number of COSA-1 and RAD-51 foci at late pachytene in him-8 and zim-2 mutants: (A) Representative images of GFP::COSA-1 in indicated strains. The numbers in the panels indicate the number of GFP::COSA-1 foci. Scale bars, 5µm. (B) Graphs showing the quantification of GFP::COSA-1 foci/nucleus in the late pachytene stage in each strain. N-value, WT = 6 gonads, 256 cells; brc-1 mutants = 10 gonads, 267 cells; him-8 mutants = 10 gonads, 272 cells; brc-1; him-8 double mutants = 9 gonads, 182 cells; zim-2 mutants = 18 gonads, 295 cells, brc-1; zim-2 double mutants = 18 gonads, 326 cells. (C) Representative images of RAD-51 immunostaining in the gonads. Perpendicular white lines indicate the border of each zone. Scale bars, 20 µm. (D) Illustration of the gonad divided into four zones of equal length from the transition zone to late pachytene (zone 1, transition zone; zone 2, early pachytene; zone 3, mid-pachytene; and zone 4, late pachytene). (E) Graphs showing the average number of RAD-51 foci per nucleus in different zones. Error bars represent the standard error of the mean. Asterisks indicate statistical difference (*p=0.01732, **p<0.00001, two-tailed Mann-Whitney test). N-value, WT = 3 gonads, 250, 235, 138, and 90 cells per zone. him-8 mutants = 3 gonads, 177, 206, 147, and 97 cells per zone. brc-1; him-8 mutants = 4 gonads, 264, 224, 167, and 96 cells per zone.