Picture from Power KM et al. (2024) MicroPubl Biol "osm-5p- driven fluorophores are differentially expressed in ...."

Figure 1. osm-5p-driven soluble GFP differs in brightness in ccpp-1Δ and nekl-4Δ mutant ciliated neurons: a. Widefield images of soluble osm-5p::GFP in the phasmid soma, sum intensity projections. Scale bar = 5 µm. b. Quantification of mean fluorescence intensity and integrated density of osm-5p::GFP fluorescence in the phasmid soma. au = arbitrary unit. Mean ± SEM; * indicates p ≤ 0.05, ** indicates p ≤ 0.01, *** indicates p ≤ 0.001, **** indicates p ≤ 0.0001 by Kruskal-Wallis one-way ANOVA with post hoc Dunn's correction for multiple comparisons. n is indicated above each genotype. Black indicates significance relative to WT, red indicates significance relative to ccpp-1Δ.

Picture from Pohl F et al. (2024) MicroPubl Biol "Pharmacological inhibition of acetylcholinesterase improves the locomotion ...."

Figure 1. Pharmacological acetylcholinesterase inhibition ameliorates mutant ATXN3-medited motor defects in a SCA3 C elegans model:A: neostigmine (0.01-10.0 mM) shows no major toxic effects in C. elegans. Toxicity was assessed using the food clearance assay. The optical density of the OP50 suspension with neostigmne-treated animals (N2) at the concentrations depicted, was measured daily. The mean OD was calculated for each day from five samples and plotted over time. Control DMSO (1%) corresponds to drug vehicle and DMSO at 5% was used as positive (toxic compound) control. B: Locomotion defective behaviour of AT3q130 animals, comparison between treated (neostigmine 0.01-10 mM) and untreated animals in comparison to wild type (N2) and AT3q14 controls. Statistical significant difference determined using One-way ANOVA and Bonferroni's multiple comparison analysis compared to AT3q130 control: ***p≤0.001 (decreased motility); *p≤0.05, ***p≤0.001; n=5 C: Locomotion defective behaviour of AT3q130 animals, comparison between treated (neostigmine 0.1-10 µM) and untreated animals in comparison to wild type (N2) and AT3q14 controls. Statistical significant difference determined using One-way ANOVA and Bonferroni's multiple comparison analysis compared to AT3q130 control: ***p≤0.001, **p≤0.01, n=5 D: Locomotion defective behaviour of AT3q130 animals compared to double mutant AT3q130; ace-1 and WT and ace-1 control. Statistical significant difference determined using One-way ANOVA and Bonferroni's multiple comparison analysis: ***p≤0.001, ns-not significant; n=5 E: Locomotion defective behaviour of AT3q130 animals compared to double mutant AT3q130; ace-2 and WT and ace-2 control. Statistical significant difference determined using One-way ANOVA and Bonferroni's multiple comparison analysis: *p≤0.05, **p≤0.01, ns-not significant; n=4

Picture from Schmidt, Minna Y. et al. MicroPubl Biol

Figure 1. 15-20 mins of swimming exercise (Ex) decreases native human &#x237A;-synuclein protein species: A. 66-<480 kDa range proteins in worms exercised for 15-20 min. on Day 1 of adulthood are significantly reduced compared to control (un-treated) and 15-20 min food restricted (FR) worms (***p≤0.0001, **p≤0.0088). These proteins are also significantly reduced for food restricted versus control (un-treated) worms (*p≤0.0116.) B. Representative Blue Native (BN) page western blot showing the protein profile of control (un-treated), Ex, and FR worms, demonstrating a reduction in proteins in 66-<480 kDa, 720 kDa, and well proteins. C. 720 kDa proteins in Ex worms are significantly reduced compared to control (un-treated) worms and almost significantly reduced compared to FR worms (***p≤0.0008, p=0.0548.) 720 kDa proteins are not significantly reduced in FR worms (p=0.4301). D. Well proteins in Ex worms are significantly reduced compared to control (un-treated) and FR worms (***p≤0.0002, ****p<0.0001). Well proteins are also significantly reduced in FR worms as compared to control (un-treated) worms (*p≤0.0144). N= 8, n=25 per replicate for A-D. E. Thrashing assay results on Day 2 show that worms previously exercised for 15-20 min on Day 1 have a significantly higher thrashing speed at 3 min compared with control (un-treated) (*p≤0.0250) or FR worms (**p≤0.0027). F. Thrashing assay results on Day 2 at 20 mins show that control (un-treated) worms have attained a higher thrashing rate, matching that of Ex worms and are significantly faster than FR worms (****p<0.0001). Ex worms maintain approximately the same thrashing speed as at 3 mins, which is also significantly higher than for FR worms (***p≤0.0003). FR worms do not see an increase in thrashing speed from 3 mins to 20 mins. G. Thrashing assay results on Day 4 demonstrate that at 3 mins, all three treatment groups display approximately the same lower thrashing speed as compared to Day 2 results. H. Thrashing assay results on Day 4 at 20 mins show that Ex worms have a significantly higher thrashing rate than control (un-treated) worms (*p≤0.0371) and an almost significant trend towards a higher thrashing rate compared with FR worms (p=0.0673). All worms show an improvement in thrashing rate compared to their speed at 3 mins; Ex worms show an average thrashing speed similar to that of control (un-treated) worms on Day 2. N=3, n=~25 worms per replicate for E-H. I-N. Representative confocal images of &#x237A;-synuclein puncta (green) after background subtraction was applied for I. control (un-treated) worms; K. Ex worms; and M. FR worms, and with calculated Imaris surfaces (pink) for J. control (un-treated) worms; L. Ex worms; N. FR worms. O. Heat map showing individual surface areas (SA's) for each puncta for each worm across all biological replicates normalized to respective controls (right Y-axis). Total puncta count for all worms combined is shown on left Y-axis. Exercise shows a higher concentration of smaller sized puncta shown in dark blue (smaller than 2 units as seen in legend.)

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)).

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)).

Picture from Le Bot N et al. (2003) Curr Biol "TAC-1, a regulator of microtubule length in the C. elegans embryo."

(B) Still images from a time-lapse movie of an embryo expressing GFP:TAC-1. The pictures show GFP:TAC-1 behavior from prophase in the 1-celled embryo (just after pronuclear-centrosome rotation) to the 2-cell stage. (0 s) prophase, (+85 s) metaphase, (+130 s) anaphase, (+140 s) telophase, (+700 s) early 2-celled embryo, (+805 s) interphase 2-celled embryo. The scale bar represents 10 um.(C) GFP:TAC-1 around the chromosomes on a metaphase plate of a multicellular embryo. The scale bar represents 5 um.

Picture from Dammermann A et al. (2008) J Cell Biol "SAS-4 is recruited to a dynamic structure in newly forming centrioles that is ...."

(A) High-resolution images of GFP:SAS-6 recruited to RFP-labeled sperm centrioles in embryos generated using the mating scheme in Fig. 1 A. Times (in seconds relative to cytokinesis onset) correspond to meiosis (-1,470 to -1,370 s), early (-1,180 to -1,156 s) and mid (-1,005 to -972 s) S phase, and late prophase (-281 to -267 s). (B) Normalized individual measurements of the integrated GFP:SAS-6 intensity coincident with sperm centriolar RFP signal.

Picture from Sandhu, Anjali et al. (2021) MicroPubl Biol

Figure 1. Transcription factors LIR-1 and PDTF-1 regulate permeability of C. elegans cuticle.: Hoechst 33258 permeability assay in (A) empty vector control (EV), pdtf-1(T26A8.1), and lir-1 RNAi in WT animals. Dashed line outlines the pharynx. Scale bar, 50 μm. n=3; N≥15. Kaplan Meier survival curves of (B) EV control, pdtf-1, and lir-1 RNAi animals against 20 mM PQ. n=3; N≥50. Percent paralyzed of EV, lir-1, and pdtf-1 RNAi in WT animals upon (C) 30 minutes exposure to 125 μM levamisole and, (D) 8 minutes exposure to 50 μM Ivermectin (IVM). n=3; N≥30. Real time PCR analysis of transcripts for collagens and collagen processing enzymes upon (E) lir-1 RNAi and (F) pdtf-1 RNAi in WT animals compared to EV control. Pcol-19::GFP expression in (G) EV, pdtf-1, and lir-1 RNAi animals. Areas with disruption of annuli and furrows are marked. Error bars indicates SEM. *, p≤ 0.05; **, p≤ 0.005; ***, p≤ 0.0005; ns-not significant, significance based on Student's t test and Mantel Cox test for histograms and survival curves respectively. p value for survival curves are indicated next to genotypes.

Picture from Driesschaert, Brecht et al. (2022) MicroPubl Biol

Figure 1.(A) Schematic representation of the research objective. The Q system is applied to establish mNeonGreen (mNG) expression and secretion (through the SEL-1 N-terminal signal peptide, SEL-1SP) from C. elegans neurons, which will end up being endocytosed and degraded by the coelomocytes. In presence of quinic acid (QA), the transcriptional suppressor (QS) is sequestered, leaving the transcriptional activator (QF) bound to the enhancer sequence (QUAS), enabling expression of mNeonGreen. Absence of QA permits interaction of QS with QF, blocking transcription (not included in diagram). (B) Coelomocyte-specific reporter signal of animals with varying QF/QS ratios, vs wild type (WT). The QF/QS ratios indicated on the X-axis refer to the ratios of the corresponding constructs in the injection mix. Quinic acid absence: white box; presence: grey box. Tukey style box plots: box from Q1 to Q3, line at median value, whisker boundaries at 1.5 interquartile range; dots are individual data points beyond that range. Statistical significance as per type III ANOVA with Bonferroni corrected p-value indications ** padj ≤ 0.005, * padj ≤ 0.05 and 'NS' padj > 0.05. (C) Percentage of worms showing a green fluorescent signal in the target tissue/cells (body wall muscle, ventral nerve cord or coelomocytes) for animals with the Q system expressed in body wall muscle (BWM) or ventral nerve cord (VNC), and a QUAS-only coelomocyte-targeted reporter strain (QUAS), respectively. For wild-type animals (WT), all three tissue types were considered. Quinic acid absence: white bar; presence: grey bar. Numbers between parentheses are number of scored worms. Statistical significance as per exact binomial tests with p-value indications ** padj ≤ 0.005, * padj ≤ 0.05 and 'NS' padj > 0.05.

Picture from Shaye DD et al. (2005) Development "LIN-12/Notch trafficking and regulation of DSL ligand activity during vulval ...."

(C) A GFP::ALX-1 translational reporter is highly and widely expressed (data not shown), and is seen in all the VPCs. This is a lateral view, with AJM-1 (red) detected by immunofluorescence, while native fluorescence of fixed GFP::ALX-1 is visualized without the need for anti-GFP staining. Note that GFP::ALX-1 appears to accumulate in vesicular structures (arrowheads) that are probably LE/MVEs.