Picture from Kim YI et al. (2004) J Mol Biol "Transcriptional regulation and life-span modulation of cytosolic aconitase ...."

I-L, ftn-2::gfp expression worms observed under DIC (I and K) and fluorescent microscope (J and L). Transgenic animals show GFP expression in pharyngeal muscle and hypodermis of wild-type on normal NGM plate. ftn-2::gfp expression worms on FAC plate were not much induced (compare IJ with KL). The bar represents 100 um.

Picture from Kim YI et al. (2004) J Mol Biol "Transcriptional regulation and life-span modulation of cytosolic aconitase ...."

Figure 4. Expression profiles of ferritins. A-H, ftn-1::gfp expression worms observed under DIC (A, C, E and G) and fluorescent microscope (B, D, F and H). Transgenic animals show GFP expression in the intestine of wild-type (A and C) and ftn-2(ok404) (E and G) on normal NGM plate. I-L, ftn-2::gfp expression worms observed under DIC (I and K) and fluorescent microscope (J and L). Transgenic animals show GFP expression in pharyngeal muscle and hypodermis of wild-type on normal NGM plate. ftn-1::gfp expression worms on FAC containing plates show much stronger GFP expression and bright dotted pattern (compare AB with EF and CD with GH). ftn-2::gfp expression worms on FAC plate were not much induced (compare IJ with KL). ftn-1::gfp expression level was strongly increased in ftn-2(ok404) (compare EF with GH). ftn-2::gfp expression level was not much induced in FAC (compare IJ with KL). The bar represents 100 um.

Picture from Hobert O et al. (1997) Neuron "Regulation of interneuron function in the C. elegans thermoregulatory pathway ...."

Figure 4. Expression Analysis of the ttx-3 Gene. (A) Expression pattern analysis of ttx-3 in wild-type animals after hatching. The schematic drawing of the AIY interneuron is adapted from White, et al. 1986.(B) Embryonic expression pattern of ttx-3. The white arrow points to the AIY cells in late embryos. We believe the additional eight staining cells in the embryos to be head muscles, due to their characteristic morphology at late embryonic stages. Expression of ttx-3 in these muscles cells is highly dynamic and developmentally regulated: in 1.5- and 2-fold embryos, the complete set of ten cells stains consistently for GFP, while in embryos just before hatching, only 47% of animals show muscle staining in addition to AIY staining (as shown in the left photograph; the remaining 53% of animals show staining in AIY only; sample size, 106 animals). Muscle staining perdures but further decreases throughout early larval stages (6% of animals show muscle staining + AIY staining; 94% show AIY staining only; sample size, 279 animals), while in late stage larvae and adults, ttx-3 expression is exclusively confined to the pair of AIY interneurons. We cannot rule out that the dynamic expression pattern in muscle reflects a lack of a promoter element in our ttx-3-GFP expression construct.