Figure 9. Conservation of GLP-1 regulation in C. briggsae. (A) C. briggsae extruded germline stained with Cb-GLP-1 antibodies. Distal end is positioned to left. White arrowheads, GLP-1 staining. (B) C. briggsae embryos stained with Cb-GLP-1 antibodies. Left, two-cell embryo; right, four-cell embryo. Blastomeres are labeled. Open white arrowhead, boundary between ABa and ABp; white arrow, boundary between EMS and P2. (C and D) RNA in situ of C. briggsae germlines and embryos using single-stranded
Cb-glp-1 cDNA probes. GL, germline; EMB, embryos; INT, intestine. (C) Antisense probe. Distal and proximal ends of germlines are labeled. Black arrowhead, internal germline staining. Top inset: one-cell and six-cell embryos. Bottom inset: internal germline staining. (D) Sense probe.