Figure 7. Expression patterns generated from a GFP-tagged Ce-Sema-2a reporter construct. All animals shown contain the
mab-20 translational fusion reporter transgene, evIs102a [
mab-20::gfp(evpPRII.14);
rol-6(
su1006)] and
him-5(
e1490). The photomicrographs to the right are epifluorescence images of the same animals shown with DIC optics on the left. Each animal is shown in a lateral perspective with anterior to the left. (A) An embryo at the comma stage (following completion of ventral enclosure). (B) Ubiquitous expression of the
mab-20::GFP reporter throughout the surface cells of the embryo shown in A, and in deeper focal planes (C). Arrows in A and B point to a seam cell. (D,E) A third larval-stage male at about 30 hours after hatching. Expression of
mab-20::GFP is observed in the R(n) cells (yellow arrows indicate three examples) that give rise to the R(n).p cells and in turn, the ray precursor clusters. (F-I) An early to mid-L4 stage male at about 35 hours after hatching. The R(n).p cells (arrows in F and G) express
mab-20::GFP, as do the ray precursor clusters (arrows in H and I point to a single cluster}, which are the R(n).p descendents and are situated in a deeper focal plane. Scale bars are 10 um.