Picture from Rose S et al. (2007) Mol Biol Cell "Caenorhabditis elegans intersectin: a synaptic protein regulating ...."

(A-D) Worms (wild type [A-C]; itsn-1(ok268) [D]) were stained with anti-ITSN-1 Ab (red). Worms are in lateral view, head to the left. NR, nerve ring (blue line in D); VC, ventral cord; DC, dorsal cord. Bar, 10 um (all panels).

Picture from Rose S et al. (2007) Mol Biol Cell "Caenorhabditis elegans intersectin: a synaptic protein regulating ...."

(E and F) Epifluorescence of transgenic worms carrying the pitsn-1:ITSN1::GFP array. Worms are in ventral (E) and lateral views (F), head to the left. SC, sublateral cords; C, commissures. Bar, 10 um (all panels).

Picture from Rose S et al. (2007) Mol Biol Cell "Caenorhabditis elegans intersectin: a synaptic protein regulating ...."

Figure 2. Presynaptic localization of ITSN-1. (A-D) Worms (wild type [A-C]; itsn-1(ok268) [D]) were stained with anti-ITSN-1 Ab (red). Worms are in lateral view, head to the left. NR, nerve ring (blue line in D); VC, ventral cord; DC, dorsal cord. (E and F) Epifluorescence of transgenic worms carrying the pitsn-1:ITSN1::GFP array. Worms are in ventral (E) and lateral views (F), head to the left. SC, sublateral cords; C, commissures. (G and H) Immunolocalization of ITSN-1 in the nIs52 strain (G; carrying the punc-25:SNB-1::GFP array) or in the oxIs22 strain (H; carrying the punc-49:UNC-49::GFP array). Red, ITSN-1; green, GFP epifluorescence. Dorsal cord is shown. Worms are in lateral view, head to the left. Bar, 10 um (all panels).

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)).

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)).

Picture from Le Bot N et al. (2003) Curr Biol "TAC-1, a regulator of microtubule length in the C. elegans embryo."

(B) Still images from a time-lapse movie of an embryo expressing GFP:TAC-1. The pictures show GFP:TAC-1 behavior from prophase in the 1-celled embryo (just after pronuclear-centrosome rotation) to the 2-cell stage. (0 s) prophase, (+85 s) metaphase, (+130 s) anaphase, (+140 s) telophase, (+700 s) early 2-celled embryo, (+805 s) interphase 2-celled embryo. The scale bar represents 10 um.(C) GFP:TAC-1 around the chromosomes on a metaphase plate of a multicellular embryo. The scale bar represents 5 um.

Picture from Dammermann A et al. (2008) J Cell Biol "SAS-4 is recruited to a dynamic structure in newly forming centrioles that is ...."

(A) High-resolution images of GFP:SAS-6 recruited to RFP-labeled sperm centrioles in embryos generated using the mating scheme in Fig. 1 A. Times (in seconds relative to cytokinesis onset) correspond to meiosis (-1,470 to -1,370 s), early (-1,180 to -1,156 s) and mid (-1,005 to -972 s) S phase, and late prophase (-281 to -267 s). (B) Normalized individual measurements of the integrated GFP:SAS-6 intensity coincident with sperm centriolar RFP signal.

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Fig 3. UNC-44 acts upstream of UNC-33. Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)). (C) Overexpression of UNC-33(S) suppresses unc-44(lf) phenotypes. Each experiment was performed with N>200 animals at least three times. For transgenic animals the results shown here are generated from at least three independent lines. Data are shown as mean +- SD. Student's t-test, ** p<0.01. Scale bar: 10 um.

Picture from Ellefson ML et al. (2009) Mol Biol Cell "Kinesin-1 and cytoplasmic dynein act sequentially to move the meiotic spindle to ...."

Images of mCherry-histone (top row) and GFP: DHC-1 (bottom row) within a wild-type meiotic embryo are shown from a representative time-lapse sequence. The cortex has been highlighted in each image for clarity. 0 s is the start of rotation. GFP:DHC-1 was faintly localized across the meiotic spindle before spindle shortening (T = -120 s). 45-30 s before the start of spindle rotation, DHC-1:GFP began to accumulate on meiotic spindle poles and remained on meiotic spindle poles after spindle rotation (T = +60 s).

Picture from Madhu Bhoomi J et al. (2019) MicroPubl Biol

Figure 1. Effects of S. marcescens and S. epidermidis on egg laying and brood size in wild-type and dbl-1(nk3) populations. (A) Comparison of mean eggs laid between wild-type (WT) and dbl-1(nk3) populations fed on E. coli OP50, S. marcescens, or S. epidermidis. The counts of number of eggs laid were reported only for plates with live animals. Error bars represent SEM. n = 7-10 surviving animals each. (B) Comparison of total brood size between wild-type and dbl-1(nk3) populations fed on E. coli OP50, S. marcescens, or S. epidermidis. Total brood sizes of animals that desiccated were censored. Error bars represent SEM. n = 7-10 surviving animals each. Within each genetic background, significant differences between the E. coli OP50-fed control and the pathogen-fed populations are marked with asterisks (*, p< 0.05, **, p< 0.001).