Picture from Ren P et al. (1996) Science "Control of C. elegans larval development by neuronal expression of a TGF-beta ...."

Figure 3. daf-7p::gfp expression in ASI neurons. (A to C) Wild-type background. (A) L1 larva; (B) dauer larva induced by pheromone; (C) recovering dauer larva about 8 hours after transfer to food at 25°C. (D) daf-7(n696) mutant background. Daf-c dauer larva, 20°C. g indicates gut autofluorescence. Arrowhead in (B) indicates the expected position of ASI cell bodies. Bars, 10 μm. (A) and (D) are three-dimensional confocal images. Faint fluorescence is apparent in the ASI axons and dendrites. (E) Diagrammed positions of the pharynx and cell bodies of ASI and neighboring neurons used for reference (one side shown). (F) GFP expression during dauer entry and recovery at 25°C. Preparation of crude pheromone extract and induction of dauer formation were as described (3). An average of 70% dauer larvae were induced with 100 μl of pheromone extract. A culture without pheromone was used as a control. Pheromone-induced dauer larvae were transferred to fresh food for recovery. PD1 and PD2 are the post-dauer equivalent of L3 and L4, respectively. Data are means ± SD for three replicates. About 20 animals were examined for each replicate.

Picture from Ren P et al. (1996) Science "Control of C. elegans larval development by neuronal expression of a TGF-beta ...."

Figure 2. Developmental Northern (RNA) analysis of daf-7 gene expression. RNA was isolated from well-fed synchronous populations (16) of wild-type eggs, L1s, L2s, L3s, L4s, adults, and pheromone-induced L2ds at 20 C (3). Approximately 20 ug of poly(A)+ RNA from each stage was fractioned on a 1% agarose-6% formaldehyde gel. A nitrocellulose blot was probed with the full-length daf-7 cDNA under high-stringency conditions (6 saline sodium phosphate EDTA, 1% SDS, 10 ug of tRNA per milliliter at 65 C) and washed at high stringency [once with 2 saline sodium citrate (SSC), 0.5% SDS for 15 min at room temperature, then twice with 0.1 SSC, 0.5% SDS for 15 min at 65 C]. The blot was exposed with an intensifying screen at 80 C for 7 days. An autoradiogram of the blot probed with an actin cDNA under the same conditions was exposed for 3 hours to indicate loading in each lane.

Picture from Barriere A et al. (2012) PLoS Genet "Coevolution within and between regulatory loci can preserve promoter function ...."

Figure 1. The expression pattern of unc-47 is conserved despite divergent regulation. (A) Both C. elegans and C. briggsae promoters fused upstream of GFP in their endogenous trans-regulatory environments drive expression in all 26 GABAergic neurons (green). However, the C. briggsae promoter placed in the C. elegans trans environment additionally drives expression in SDQR and SDQL (blue). (B) Those cells were identified as SDQR/L based on their position and their characteristic projections. (C) For each combination of promoter and trans-regulatory environment, expression in SDQR and SDQL is presented. C. elegans is represented by straight lines, C. briggsae by wavy lines. Frequency of expression is represented by the width, and intensity of expression relative to D-type neurons by the height of black boxes. Number of individuals expressing and total number of individuals scored is indicated underneath. Measurements for independent strains are given in Figure S1. Differences in frequency of expression in SDQR are significant for all comparisons: C. elegans and C. briggsae promoters in C. elegans trans environment (p = 8.1610212), C. elegans and C. briggsae promoters in C. briggsae trans environment (p = 1.561028), C. elegans promoter in C. elegans and C. briggsae trans environments (p = 4.8610211), C. briggsae promoter in C. elegans and C. briggsae trans environments (p = 2.4610214). (D) Interpretation of the results presented in panel C. Both C. elegans and C. briggsae promoters in their endogenous trans environments drive low levels of expression in SDQR and SDQL, while disruption of the endogenous interactions either drives high levels of expression (C. briggsae promoter in C. elegans) or abolishes expression (C. elegans promoter in C. briggsae).

Picture from Beaster-Jones L et al. (2004) J Mol Biol "DNA binding and in vivo function of C.elegans PEB-1 require a conserved FLYWCH ...."

Figure 2. Cb-PEB-1 and Ce-PEB-1 are expressed in similar pattern. Fluorescence micrographs of 1.5-fold stage C. briggsae (A) and C. elegans embryos (B) stained with C. elegans PEB-1 antibodies (anterior is left). Staining is most abundant in pharyngeal nuclei in both C. briggsae and C. elegans (brackets). Fainter staining is visible in hypodermal cells and the hindgut in C. elegans and C. briggsae, although all staining was generally weaker in C. briggsae.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(C, C') Pgrl-2::GFP is expressed in the excretory duct and pore cells.

Picture from Barkoulas M et al. (2016) PLoS Genet "Evolution of New cis-Regulatory Motifs Required for Cell-Specific Gene ...."

(B-E) smFISH using a lin-3 probe in C. elegans N2 (data from [2]) (B), C. briggsae AF16 (C), C. angaria RGD1 (D) and O. tipulae CEW1 (E). Red arrow marks the position of the anchor cell.

Picture from Hobert O et al. (1999) J Cell Biol "A conserved LIM protein that affects muscular adherens junction integrity and ...."

(A-C): UNC-97::GFP localization in live worms at embryonic stage 300 min (A), 400 min (comma stage) (B), and 500 min (threefold pretzel stage) (C). (A'-C') Corresponding DIC micrographs. White arrows in C, a muscle nucleus and dense bodies, respectively. Note UNC-97::GFP localization to the periphery of nuclei in A-C.

Picture from Vazquez-Manrique RP et al. (2007) Genomics "The frataxin-encoding operon of Caenorhabditis elegans shows complex ...."

(a-c) Expression of F59G1.1::gfp in pharynx and gut (a), anal cells (b), and spermatheca (sph) (c).

Picture from Cui Y et al. (2009) J Biol Chem "Cadmium induces retinoic acid signaling by regulating retinoic acid metabolic ...."

bcmo-1::GFP expression in post-embryonic C. elegans. Images of untreated C. elegans (A, C, and E). L1 (A) and L4 (C) larvae are at 400x and 200x magnification, respectively. Adult C. elegans (E) is a composite of images acquired at 200x magnification. Arrowhead, indicates the location of a bcmo-1::GFP expressing embryo in a gravid adult nematode.

Picture from Loria PM et al. (2003) Curr Biol "Two neuronal, nuclear-localized RNA binding proteins involved in synaptic ...."

(B and C) Expression of promoter fusions in embryos at the (B) 300-cell stage and in (C) L1 larvae.