E: Images of CEH-84 protein fusion reporter, PHX2811 (allele
syb2811), generated by insertion of gfp sequence by CRISPR/Cas9. On the left, embryonic GFP expression looks diffuse and non-nuclear. On the right, no detectable GFP in L4 hermaphrodites. Solid white lines outline the worm structures, dashed lines highlight the intestinal GFP autofluorescence, and the white V marks the vulva. Using a ~2kb promoter fusion, postembryonic intestinal expression was observed by McKay et al., 2003 and weak embryonic expression was noted by Hench at al., 2015.