Picture from Reddy KC et al. (2017) Curr Biol "An Intracellular Pathogen Response Pathway Promotes Proteostasis in ...."

(A and B) Confocal fluorescence images of adult animals with fosmid transgenes expressing (A) PALS-22::GFP and (B) CUL-6::GFP from endogenous promoters.

Picture from Reddy KC et al. (2017) Curr Biol "An Intracellular Pathogen Response Pathway Promotes Proteostasis in ...."

(A and B) Confocal fluorescence images of adult animals with fosmid transgenes expressing (A) PALS-22::GFP

Picture from Reddy KC et al. (2017) Curr Biol "An Intracellular Pathogen Response Pathway Promotes Proteostasis in ...."

Figure 4. PALS-22 Is Broadly Expressed and Functions in the Intestine to Regulate IPR Expression and Thermotolerance(A and B) Confocal fluorescence images of adult animals with fosmid transgenes expressing (A) PALS-22::GFP and (B) CUL-6::GFP from endogenous promoters.(C) qRT-PCR measurement of IPR gene expression in animals with tissue-specific expression of a wild-type pals-22 cDNA driven by endogenous (pals-22),intestinal (vha-6), neuronal (unc-119), or hypodermal (dpy-7) promoters in a pals-22(jy1) mutant background. Results are shown as the fold change relative to wildtype control and are the average of two independent biological replicates. Error bars are SD. *p < 0.05; n.s., not significant by Student's t test.(D) Survival of animals with pals-22 tissue-specific rescue after 2 hr heat shock treatment at 37C followed by 24 hr at 20C. Strains were tested in triplicate.Fraction alive indicates mean survival among the triplicates; errors bars are SD. **p < 0.01; *p < 0.05; n.s., not significant by Student's t test. Heat shock assay wasrepeated three independent times with similar results; data from a representative experiment are shown.(E) Measurement of intestinal cul-6 mRNA transcripts using single-molecule fluorescence in situ hybridization (smFISH). Graph is a compilation of three independent biological replicates. Each symbol represents the smFISH spot count from the four anteriormost intestinal cells of an individual worm. Statistical analysiswas performed using one-way ANOVA. ****p < 0.0001; *p < 0.05; n.s., not significant by one-way ANOVA.See also Figure S4.

Picture from Reddy KC et al. (2004) Cell "C. elegans HIM-17 links chromatin modification and competence for initiation of ...."

Figure 5. HIM-17::GFP Localizes to Chromatin in Germline Nuclei. Immunolocalization of HIM-17::GFP in germline nuclei of him-17(ok424) hermaphrodites carrying a rescuing him-17::gfp transgene.(A) Low magnification images showing premeiotic nuclei and nuclei progressing through all stages of meiotic prophase, oriented from left to right. HIM-17::GFP (green) is present in all nuclei in the germ line, and is localized mainly on chromatin in all stages prior to diakinesis, when HIM-17::GFP is detected throughout the nucleoplasm. Scale bar equals 4 μm.(B) High magnification images of pachytene stage nuclei, showing that HIM-17::GFP is concentrated on chromatin. In the merged image, HIM-17::GFP is shown in green and DAPI-stained chromosomes are in blue. Images are projections approximately halfway through 3D data stacks of whole nuclei; scale bar equals 2 μm.

Picture from Lee J et al. (2004) J Mol Biol "Opposing functions of calcineurin and CaMKII regulate G-protein signaling in ...."

Calcineurin A expression in hypodermal cells was observed by (A) GFP expression as well as (B) immunostaining.

Picture from Pauli F et al. (2006) Development "Chromosomal clustering and GATA transcriptional regulation of ...."

Intestinal expression of D2030.5 (mce-1). Expression is shown in the intestine as well as other tissues.

Picture from Sze JY et al. (2003) Proc Natl Acad Sci U S A "Activity of the Caenorhabditis elegans UNC-86 POU transcription factor ...."

In wild-type animals, the lin-11::GFP is expressed in the AIZ interneurons as well as several other head neurons.

Picture from Nelson MD et al. (2011) PLoS Genet "A bow-tie genetic architecture for morphogenesis suggested by a genome-wide ...."

RME-8::GFP localizes to the edge of detaching and retracting tail tip cells as puncta (depicted as dark green dots in the schematic).

Picture from Meissner B et al. (2011) PLoS One "Determining the sub-cellular localization of proteins within Caenorhabditis ...."

Sub-cellular localization pattern for F42C5.9. F42C5.9 is present in the cell membrane, as well as filament-like structures and dense body-like structures. Bar 10 um.

Picture from Stitzel ML et al. (2007) Curr Biol "Regulation of MBK-2/Dyrk kinase by dynamic cortical anchoring during the ...."

(C) Images of live embryos expressing GFP fusions as indicated.