Picture from Nehrke K et al. (2002) J Biol Chem "The NHX family of Na+-H+ exchangers in Caenorhabditis elegans."

Q and R, the nhx-9 promoter drove GFP expression in the excretory cell (ec) as well as in the phasmid sensory organs in the tail (phm) and the posterior intestine (int; AI and AJ).

Picture from Hsu TY et al. (2010) Curr Biol "Engulfment of apoptotic cells in C. elegans is mediated by integrin alpha/SRC ...."

(Aa-Aj) INA-1::GFP is enriched in the pseudopods extending around apoptotic cells. Time-lapse images of INA-1::GFP (Aa-Ae) and differential interference contrast (DIC; Af-Aj) during cell corpse internalization in an embryo expressing Pced-1ina-1::gfp are shown. 0 min indicates the time point at which INA-1::GFP was first seen to accumulate at the contact site with C3. Arrowheads indicate the apoptotic cell (C3); arrows indicate the engulfing cell (ABplaapppp). Scale bar in (Aa) represents 5 um.

Picture from Kazatskaya A et al. (2017) Genetics "Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the ...."

(B) Representative images of the indicated GFP-tagged MAPK-15 reporters in wild-type worms. Constructs (shown in schematicsat right) derived from mapk-15 genomic sequences (red) under the control of mapk-15 upstream regulatory promoter sequences(Prom; orange). Strains expressing the mapk-15a::gfp DNA transgene at low levels generated by injecting worms with a low DNAconcentration (0.2 ng/ul). BB: basal body. AJ: apical junction. Cil: ciliary axoneme. Anterior is at top (head images) and left (tailimages). Scale bars: 3 um (head images) and 5 um (tail images).(C) Representative images of localization of GFP::MAPK-15c expressed under its endogenous promoter in mapk-15(gk1234)animals. BB: basal body. AJ: apical junction. Cil: ciliary axoneme. Anterior is at top (head images) and left (tail images). Scale bars:5 um.

Picture from Kazatskaya A et al. (2017) Genetics "Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the ...."

Figure S2. GFP-tagged MAPK-15 localizes to the cilia and ciliary base, and mapk-15 expression in most ciliated neurons isDAF-19-independent.(A) Representative images of mapk-15p::gfp expression in wild type (WT) and daf-19(m86);daf-12(sa204) worms. Lab: inner labialcells, quad: outer labial quadrant cells. Schematic of the mapk-15p::gfp reporter construct shows mapk-15 upstream regulatorysequences (+ first 11 bp of exon 1) fused to gfp, and the location of a putative X-box sequence. Numbers refer to genomic positionsrelative to the start codon. Anterior is at left. Scale bars: 20 um.(B) Representative images of the indicated GFP-tagged MAPK-15 reporters in wild-type worms. Constructs (shown in schematicsat right) derived from mapk-15 genomic sequences (red) under the control of mapk-15 upstream regulatory promoter sequences(Prom; orange). Strains expressing the mapk-15a::gfp DNA transgene at low levels generated by injecting worms with a low DNAconcentration (0.2 ng/ul). BB: basal body. AJ: apical junction. Cil: ciliary axoneme. Anterior is at top (head images) and left (tailimages). Scale bars: 3 um (head images) and 5 um (tail images).(C) Representative images of localization of GFP::MAPK-15c expressed under its endogenous promoter in mapk-15(gk1234)animals. BB: basal body. AJ: apical junction. Cil: ciliary axoneme. Anterior is at top (head images) and left (tail images). Scale bars:5 um.

Picture from Hsu TY et al. (2010) Curr Biol "Engulfment of apoptotic cells in C. elegans is mediated by integrin alpha/SRC ...."

Figure 2. INA-1 Recognizes Apoptotic Cells and Is Required for Their Internalization by Engulfing Cells. (Aa-Aj) INA-1::GFP is enriched in the pseudopods extending around apoptotic cells. Time-lapse images of INA-1::GFP (Aa-Ae) and differential interference contrast (DIC; Af-Aj) during cell corpse internalization in an embryo expressing Pced-1ina-1::gfp are shown. 0 min indicates the time point at which INA-1::GFP was first seen to accumulate at the contact site with C3. Arrowheads indicate the apoptotic cell (C3); arrows indicate the engulfing cell (ABplaapppp). Scale bar in (Aa) represents 5 μm.(Ba-Bc) ina-1 mutations reduce the efficiency of cell corpse internalization in engulfing cells.(Ba and Bb) MYRI::GFP and DIC images of wild-type (Ba) and ina-1(gm144) (Bb) embryos expressing Pced-1myri::gfp. Arrows indicate apoptotic cells. Scale bar in (Ba) represents 5 μm.(Bc) Percentage of apoptotic cells with a MYRI::GFP circle in the indicated genotype. Data (mean ± SD) are the average for two independent transgenic lines. Numbers in parentheses indicate the number of cell corpses scored.(Ca-Cc) INA-1(N) binds to the surface of apoptotic cells in a scrm-1-dependent manner.(Ca and Cb) INA-1(N)::GFP and DIC images of ced-1(e1735); ced-5(n1812) (Ca) and scrm-1(RNAi)ced-1(e1735); ced-5(n1812) (Cb) embryos expressing Phspina-1(N)::gfp. Arrows indicate apoptotic cells. Scale bar in (Ca) represents 5 μm.(Cc) Percentage of INA-1(N)::GFP+ cell corpses in the indicated genotype. Cell corpses fully enclosed by the INA-1(N)::GFP circle were scored as INA-1(N)::GFP+. Each sample point represents the data from 12 embryos. Data are shown as mean ± SD.

Picture from Topalidou I et al. (2017) PLoS Genet "Dopamine negatively modulates the NCA ion channels in C. elegans."

(D) grk-2 is expressed in head acetylcholine neurons. Representative images of a Z-stack projection of the area around the nerve ring in the head of an animal coexpressing tagRFP fused to the GRK-2 ORFdriven by the grk-2 promoter (grk-2::tagRFP, integration yakIs19) and GFP under a head acetylcholine neuron promoter(Punc-17H::eGFP, transgene yakEx94). Anterior to the left. Because Punc-17H::GFP is highly expressed and diffusethroughout the cell but grk-2::tagRFP is dimmer and localized only in the cytoplasm, their coexpression is hard to see in themerged image. For this reason, we have circled the cells where there is coexpression. Scale bar: 10 um.

Picture from Kitaoka S et al. (2013) PLoS One "FGT-1 is a mammalian GLUT2-like facilitative glucose transporter in ...."

Figure 5. Cellular and subcellular localizations of FGT-1 in C. elegans. The fgt-1::egfp fusion construct under the control of the fgt-1 promoter was injected into C. elegans, and the expression of the FGT-1::eGFP fusion protein was visualized with a confocal laser microscope. A: whole animal, B: embryos inside of a parental worm, C: higher magnification image of mid-body. For each panel (A to C), GFP fluorescence was shown alone or as a merged picture with differential interference contrast (DIC) images. D-H. Immunostaining of the apical membrane marker IFB-2 for fgt-1::egfp embryos. Subcellular localizations of FGT-1::eGFP (green) and IFB-2 (red) were observed under a confocal laser microscope. D: FGT-1::eGFP, E: IFB-1 immunostaining. F: Merged image of D and E.G: The higher magnification of the white box area in F.H: Merged image of eGFP fluorescence and DIC images. I. Schematic diagram of an intestinal cell with subcellular localizations of eGFP (green) and IFG-2 (red). The potential adherent junction (AJ) is indicated by a dark box.

Picture from Dejima K et al. (2009) FASEB J "The ortholog of human solute carrier family 35 member B1 (UDP-galactose ...."

Figure 5. HUT-1 localizes to the ER and part of the Golgi in the intestine and is required for normal ER morphology. A, B) Confocal micrographs of intestinal cells expressing EGFP::HUT-1 and the ER marker mCHERRY::SP12 (A) and the Golgi apparatus marker AMAN-2::mCHERRY (B). Nuclei are indicated by 'N.' A) Top: EGFP::HUT-1 colocalized with the ER marker as well as distrib- uted as small particles adjacent to the ER. Some of these particles are indicated by arrowheads. Bottom: knockdown of hut-1 by RNAi led to aggregation and resulted in abnormal ER network morphology resembling thin ropes or strings (arrows). B) Top: EGFP::HUT-1 partially colocalized with the Golgi apparatus marker (arrowheads). EGFP::HUT-1 tended to be detected as small particles when coexpressed with AMAN-2::mCHERRY for unknown reason. Bottom: no apparent alteration in the Golgi apparatus marker was observed with hut-1 RNAi. C) Representative DIC and fluorescent images of HUT-1::EGFP under the control of hut-1 promoter show expression in an L4 larva. Scale bars = 10 um (A); 50 um (B, C).

Picture from Wilkinson HA et al. (1995) Genetics "Spatial and temporal patterns of lin-12 expression during C. elegans ...."

FIGURE2. lin-12::lacZ expression in the first phase of somatic gonadal development. (A) Schematic diagram of the Z1 and 24 lineages during the first phase of somatic gonad development (adapted from KIMBIX and HIRSH1979). Filled circle , cells that express the reporter gene; dotted circle, staining is only sometimes observed in Z1.aa and Z4.pp (the distal tip cells) or in Z1.paa or Z4.app. None of these staining patterns is found in a high percentage of animals and the reason for this variability is unknown. (R and C ) Nomarski photomicrographs of the somatic gonad of an L2 larva that has been fixed and stained for &galactosidase activity as described in MATERIALS AND METHODS. Anterior is to the left. Lateral view of an L2 animal. R and c show different planes of focus. Due to the Roller phenotype, all lin-lZ::lmZ expressing cells in the somatic gonad are visible from this angle. All four cells that have the potential to adopt the W fate, Zl.ppa, Zl.ppp, Z4.aaa and Z4.aap, express the lin-12::lacZ reporter gene at this stage. Note that the VPCs also express the lin-12 transgene at this time and these cells appear as dark spots or patches in the photographs.

Picture from Gobel V et al. (2004) Dev Cell "Lumen morphogenesis in C. elegans requires the membrane-cytoskeleton linker ...."

Figure 1. ERM-1 Is Enriched at Luminal Surfaces. Here and elsewhere, animals are viewed from the side: anterior left, posterior right, dorsal top, ventral bottom. Gray boxes in schematics (iA, iiA, and iiiA) indicate areas magnified in images beneath. (iC and iiB) Nomarski images; (iD and iF) confocal sections; (iE, iiC, iiiB, and iiiC) confocal projections.i. Intestine. (A) ERM-1 localizes to intestinal lumen (green). (B) Wild-type intestine with 20 bilaterally symmetrical cells in 9 rings (4 cells for first ring; blue), intestinal lumen (green), AJ (red); tube is rotated along longitudinal axis. (C-F) Anterior intestinal tube; apical (small arrowheads) and basal (large arrowheads) sides of tube indicated. ERM-1-GFP (D and E) is at the lumen, apical to but distinct from α-AJM-1 (yellow in [E] from integration of sections, not overlap). ERM-1-GFP also seen in excretory canal in (E) (arrow). NFM-1-lacZ (F) is localized basolaterally.ii. Excretory canals. (A) ERM-1 localizes to excretory canals (red; canals shown separated for clarity). (B and C) Anterior excretory canals: ERM-1-GFP outlines canals and also the intestinal (arrow), but not pharyngeal (arrowhead) lumen.iii. Gonad. (A) ERM-1 localizes to gonad lumina: spermathecae, uterus, vulva (blue). (B) Integration of sections through uterine-vulval connection (ventral view); vulva opens to right: ERM-1-GFP outlines eggs in uterus and vulval opening (arrowhead). (C) Vulva (large arrowhead) at higher magnification (ventrolateral view; vulva opens to right): ERM-1-GFP is separate from complex junctional α-AJM-1 pattern (yellow from integration of sections, not overlap); intestinal lumen at left (small arrowhead).