Picture from Yang Q et al. (2017) Dev Cell "The Invading Anchor Cell Induces Lateral Membrane Constriction during Vulval ...."

(M-P) Localization of the endogenous GFP::TOCA-1 reporter zh110 in wild-type (M) and unc-6(ev400) mutants (O and P) before (M and O) and after (N and P)lumen formation. (M') and (O') show yz projections of the lateral VulF membranes. The inset in (O) shows the displaced AC in the unc-6(ev400) mutant in a differentfocal plane.

Picture from Haag A et al. (2014) PLoS Genet "An in vivo EGF receptor localization screen in C. elegans Identifies the Ezrin ...."

(O) intracellular punctate expression of C11H1.3::GFP in P6.p.

Picture from Tian Y et al. (2010) Cell "C. elegans screen identifies autophagy genes specific to multicellular ...."

(M-O) The epg-5 reporter is expressed ubiquitously in embryos (N) and widely in larvae (O). EPG-5 is cytoplasmic. (M) Nomarski image of the embryo in N.

Picture from Nehrke K et al. (2002) J Biol Chem "The NHX family of Na+-H+ exchangers in Caenorhabditis elegans."

O and P, transgenic nematodes expressing nhx-8::GFP in the lateral seam cells (sc).

Picture from Ono K et al. (2015) Mol Biol Cell "UNC-87 isoforms, Caenorhabditis elegans calponin-related proteins, interact ...."

(O-W) Expression ofGFP in hermaphrodite gonads with no transgene (O-Q), Punc-87A::GFP (R-T), or Punc-87B::GFP (U-W) was examined by immunostaining of dissected gonads for GFP (O, R, and U) and MYO-3 as a marker of myoepithelial sheath (P, S, andV). Merged images are shown in Q, T, and W (green, GFP; red, MYO-3; blue, DNA [not shown as individual images]). Scale bars, 100 um (whole worms; A, B, I, and J), 20 um (magnified tissues; C-H,K-N), 50 um (dissected gonads; O-W).

Picture from Nishi Y et al. (2008) Development "Polo kinases regulate C. elegans embryonic polarity via binding to DYRK2-primed ...."

PLK-1 cytoplasmic asymmetry in early C. elegans embryos. Merged immunofluorescence micrographs of DAPI staining (red) with anti-PLK-1 (A-F) staining (green). (G-L) GFP fluorescence of GFP::PLK-1PBD. (O,P) GFP fluorescence of GFP::PLK-1FL (O). Closed and open arrowheads indicate the germline blastomere and its somatic sister, respectively. Stages of embryos in A-L are marked to the left; O, 2C. Scale bar: 10 μm.

Picture from Hashmi S et al. (2008) DNA Cell Biol "A Kruppel-like factor in Caenorhabditis elegans with essential roles in fat ...."

FIG. 2. Temporal pattern of klf-1 gene expression as determined by real-time PCR. Note that Ce-klf-1 transcripts are low in embryos but increased several folds in the larval stages and decreased again in adult.

Picture from Wimberly, Keon et al. (2020) MicroPubl Biol

A) Schematic of primer design approach; the deletion is red. B) Genomic map of the numr-1 and -2 deletion. C-F) Real-time PCR amplification and melt curves for inner primers (C-D) and outer (E-F) primers.

Picture from Sugimoto T et al. (2008) Exp Cell Res "Caenorhabditis elegans par2.1/mtssb-1 is essential for mitochondrial DNA ...."

(B) The expression levels of mtssb-1 during development weremonitored with real-time quantitative RT-PCR as compared with the expression level of an elongation factor eft-2. Relative ratio of each expression was calculated using the adult expression levels.

Picture from Delawary M et al. (2007) Biochem Biophys Res Commun "Molecular characterization of a novel RhoGAP, RRC-1 of the nematode ...."

Fig. 3. Real-time PCR analysis of the gene expression level of rrc-1. The mRNA values for each group were normalized with the reference gene, b-actin. Results are expressed as mean values of the group ± SD. n = 3 in each group.