Picture from Minor, Paul J et al. (2019) MicroPubl Biol

Figure 1. LRP-2 domains and phylogeny: (A) Protein domains of LRP-2, LRP-1, and Drosophila Arrow. C. elegans does not possess a true ortholog of Arrow (LRP5/6); however, it does possess multiple megalin-like proteins that contain LDLR Class A repeats, LRDR Class B repeats, and EGF-like domains that are found in varieties of low density like lipoprotein receptors such as megalin and Arrow. All domains are color-coded and drawn to approximate scale according to the SMART database. (B) An evolutionary tree based on the protein sequence of LRP-1 and LRP-2 in nematodes and megalin in Drosophila melanogaster. Based on sequence similarity, position in the genome, and clustering, it appears that LRP-2 is the result of a recent duplication in Caenorhabditis. (C) Within Caenorhabditis, LRP-1 orthologs cluster together and LRP-2 orthologs cluster. Pristionchus pacificus is used as the outgroup.

Picture from Eren, Guniz Goze et al. MicroPubl Biol

Figure 1. Integration of transgenes into the P. pacificus genome(A) Plasmid carrying Ppa-egl-20p::TurboRFP including codon optimisation and the insertion of native P. pacificus introns. (B)Cartoon schematic of UV induced integration methodology. Successful integration occurred in 2 / 250 animals after 0.050 J /cm2 UV exposure increasing transmission to 100%. (C) Confocal image of Ppa-egl-20p::TurboRFP with expression in several tail neurons in a successful UV integrated line. Scale bar = 25 μm. (D) Wild-type P. pacificus and an uncloned Ppa-dpy mutant generated by UV irradiation and identified in our screen. Scale bar = 250 μm.

Picture from Wang X et al. (2011) PLoS Biol "Antagonism of LIN-17/Frizzled and LIN-18/Ryk in nematode vulva induction ...."

Figure 6. Expression of Ppa-lin-17 and Ppa-egl-20 control vulva induction in P. pacificus. (A-C) J2 larval stage of wild-type P. pacificus animal. (A) Ppa-lin-17 promoter driving nuclear localized TurboRFP expression in the posterior tail. (B) Within the same animal, GFP under the control of the Ppa-egl-20 promoter. (C) Merged image of Ppa-egl-20::GFP; Ppa-lin-17::TurboRFP. (D) Ligand sequestration model. In wild type, Ppa-LIN-17 sequesters Ppa-EGL-20 in the posterior body region. In Ppa-lin-17 mutant animals, excessive Ppa-EGL-20 ligand reaches the central body region induces vulva formation in the absence of the signal from the second signaling center, the somatic gonad. A mutation in Ppa-egl-20 fully suppresses the gonadindependent vulva differentiation phenotype of Ppa-lin-17.

Picture from Le, Vivian Vy et al. MicroPubl Biol

Paralysis was assessed by body movements. Twenty worms were tested per assay and >10 replicates were included per condition. The first reading was taken at 10 minutes for the levamisole and ivermectin treatments. Error bars denote standard error of the mean. Each data set was tested for normality before testing for significance. Unpaired t-test was used to calculate for statistical significances between P. pacificus and C. elegans for betaine, levamisole, and halothane. The Wilcoxon test was used to calculate for statistical significances for ivermectin. P* < 0.05, P** < 0.01, P*** < 0.001, P**** < 0.0001.

Picture from Loer, Curtis et al. MicroPubl Biol

Figure 1: FLAG antibody-stained P. pacificus worms using the HIAR-added protocol, as described in the text. Details on generating CRISPR/Cas9-mediated FLAG-epitope tagged alleles, and precise sites and developmental time courses of nuclear expression will be reported elsewhere. (A) Three Ppa-unc-3::2xFLAG larval worms (RS3506) stained with mouse anti-FLAG monoclonal antibody plus Alexa-594-conjugated anti-mouse secondary. (B) Staining of Ppa-unc-86::1xFLAG strain (RS3509) with anti-FLAG two tails (top), one head (bottom) of larve; antibodies as in A.

Picture from Noble LM et al. (2015) Curr Biol "Natural Variation in plep-1 Causes Male-Male Copulatory Behavior in ...."

Figure 3. plep-1 Expression and Phylogeny (A) Maximum intensity projection along the dorsoventral axis showing pplep-1::GFP expression in the excretory cell body, just posterior to the terminal pharyngeal bulb, and symmetric canals in a young adult hermaphrodite. (B) Lateral view of a young adult male. In addition to consistent expression in the excretory cell, fluorescence in two dorsal cells in the male tail, shown here, was observed inconsistently. (C) Phylogeny for UNC-93 domain (IPR010291) homologs. UNC-93, MFSD11, and PLEP-1 clades are highlighted (red, blue, and yellow). Unboxed genes are MFSD11 paralogs in Caenorhabditis and Pristionchus nematodes.