Picture from Tellez-Arreola, Jose L et al. (2019) MicroPubl Biol

Figure 1. Structure and effects of chrysin and its Br-derivative. A) Structure of chrysin and 7-O-(3-bromopropyl)-chrysin, B) Representative GABA-currents in presence or absence of 7-O-(3-bromopropyl)-chrysin. Percent of change of the GABA-induced current amplitude in presence of chrysin and chrysin-derivative at different concentrations. C) Speed and wavelength of the nematodes in the presence of the compounds after 3 h of exposure to 100 µM in each condition.

Picture from Ruaud AF et al. (2007) Development "The P-type ATPase CATP-1 is a novel regulator of C. elegans developmental timing ...."

Fig. 1. Mutation of catp-1 confers resistance to the nicotinic agonist DMPP. (A) Dose-response sensitivity to DMPP of wild type (WT) and catp-1(kr17) mutants. (B) catp-1 gene structure and constructs used for transgenesis experiments. Triangle, Mos1 insertion; dashes, direct repeats; arrows, inverted repeats; *, STOP codon. (C) Survival on 0.75 mM DMPP. Error bars represent s.e.m. (n≥4 independent experiments, N≥96 total individuals, two independent transgenic lines per construct tested). Pcatp-1(br)::catp-1(br): extrachromosomal array carrying the C. briggsae catp-1 genomic region; Pcatp-1(br)::catp-1(el): C. elegans catp-1 cDNA under the control of a C. briggsae catp-1 promoter. Both constructs rescued catp- 1(kr17) DMPP resistance. (D-G) catp-1 expression profile. Detection of GFP fluorescence by confocal (D) and epifluorescence microscopy (E) in transgenic larvae expressing Pcatp-1(C. briggsae)::GFP. catp-1 is expressed in the hyp (white arrowheads) and Pn.p cells of the epidermis (D) and in the duct cell of the excretory system (E-G). No GFP expression was detected in the lateral cells of the epidermis (seam cells: black arrowheads). In (G), the GFP image (E) of the duct cell was overlayed on a Nomarski picture of the same field (F) where the excretory duct is visible (black arrow). Scale bars: 20 μm (D); 5 μm (E-G). (H) Survival on 0.75 mM DMPP. Error bars represent s.e.m. (n≥3 independent experiments, N≥111 individuals, two independent lines). Expression of a dsRNA targeting catp-1 in the epidermis induces partial DMPP resistance in a sid-1(qt2) background (*P<0.05, Mann-Whitney test).

Picture from Lee RC et al. (2001) Science "An extensive class of small RNAs in Caenorhabditis elegans."

Figure 1. Northern blots of small RNA transcripts. (A through C) Total RNA from C. elegans larvae (stages L1 through L4) or from mixed stage (M) populations were blotted and probed with oligonucleotides complementary to either the 5' or 3' half of the indicated transcript (13). U6 = the same filters were probed with probe to U6 snRNA as a loading control. (A)mir-60 5' probe detects a transcript of ~65 nt. The ratio of L1 to L4 mir-60 signal, normalized to U6, is about 5:1. The mir-60 3' probe (not shown) detects a similar-sized species with a similar developmental profile. (B) mir-80 3' probe detects a ~22-nt RNA expressed uniformly at all stages. (C) mir-52 5' probe. The normalized mir-52 signal is threefold greater in the L1 versus the L3. (D) mir-1 3' probe detects a transcript of ~22 nt in total RNA from mouse (Mm) 17-day embryos, mixed-stage C. elegans (Ce), Drosophila melanogaster (Dm) mixture of embryo-larvae-pupae, and in a sample of human heart (ht) tissue. Other human tissue samples were brain (br), liver (li), kidney (ki), and lung (lu). (E)mir-1 and mir-58 probes to total RNA from mixed populations of wild-type (+) and dcr-1(ok247) (-) animals. An increase in the proportion of unprocessed ~65-nt precursor is observed in the dcr-1 RNA.