Figure 1. Knockdown of
jph-1 gene expression results in altered nervous system structure and a reduced thrashing phenotype: A. (a) and (b) AGD850 (YFP-labeled nervous system) strain of C. elegans on Nematode Growth Medium lite (NGM-lite) with OP50 Escherichia coli (E. coli) feeding strain exhibiting normal nervous system structure (observed day 3); (c) and (d) AGD850 strain of C. elegans on NGM-lite + kanamycin + isopropyl ß-D-1-thiogalactopyranoside (NKI) with
jph-1 RNAi E. coli feeding strain exhibiting altered nervous system structure due to RNAi knockdown. Orange arrows = head ganglion, blue arrows = tail ganglion, gray arrow = ventral cord. B. Detailed description of treatments, including C. elegans strains and media. C. Percent knockdown (KD%), percent viability, population density, and egg presentation for all treatments; n = number of plates observed. D. Average number of thrashes among two different strains of C. elegans (wild-type (N2) and
jph-1 knockout (VC2286)) each plated on two different types of media (NGM-lite/OP50 feeding strain; NKI/jph-1 RNAi feeding strain); error bars indicate the variation of average thrashes between two trials; * = P < 0.01 Tukey HSD Test; ANOVA P < 0.0001.
jph-1 knockout thrashing assay results replicate those of Piggott et al., 2021.