Picture from Pichler SC et al. (2000) Development "OOC-3, a novel putative transmembrane protein required for establishment of ...."

Figure 10. OOC-3 localization in two- and four-cell stage embryos. Confocal micrographs of embryos that have been stained with OOC3Ct2 to reveal the localization of OOC-3 protein and counterstained with Hoechst 33258 to reveal DNA. (a,b) Prophase of two-cell stage embryo. OOC-3 localizes to a reticular structure spanning the whole cytoplasm being enriched at the perinuclear region. Furthermore, it is slightly enriched at the cell-cell boundary (black arrowheads). (a) OOC-3, (b) DNA. (c,d) In mitosis OOC-3 localizes to the mitotic spindle. (c) OOC-3, (d) DNA. (e,f) In four-cell stage embryos OOC-3 localizes to the circumference of nuclei and to a reticular structure spanning the cytoplasm. Furthermore, the protein is enriched at the cell-cell boundaries (black arrowhead). (e) OOC-3, (f) DNA. (g,h) 0.2 µm slice taken with a Deltavisionmicroscope and deconvolved. Enlargement to show the reticular structure and outline of the nuclear envelope of OOC-3 in a prophase cell. (g) OOC-3, (h) DNA. Bar, 10 µm.

Picture from Pichler SC et al. (2000) Development "OOC-3, a novel putative transmembrane protein required for establishment of ...."

Figure 9. OOC-3 localization in one-cell stage embryos. Confocal micrographs of embryos that have been stained with OOC3Ct2 to reveal the localization of OOC-3 protein and counterstained with Hoechst 33258 to reveal the DNA. (a,b) One-cell stage embryos at fertilization. OOC-3 localizes under the cortex of the embryo. (a) OOC-3, (b) DNA. (c,d) One-cell stage embryo at P0rotation. OOC-3 localizes to a reticular structure spanning the whole cytoplasm being enriched at the circumference of nuclei and the asters. (c) OOC-3, (d) DNA. (e,f) One-cell stage embryo in mitosis. OOC-3 localizes to the mitotic spindle. (e) OOC-3, (f) DNA. (g,h) ooc-3 mutant embryo at the time of P1 rotation. Staining with antibody to OOC-3. No staining reminiscent of OOC-3 could be observed. (g) OOC-3, (h) DNA. (i,j) Peptide competition. Wild-type embryo at pronuclear migration stained with a mixture of OOC3 Ct2 and the peptide against OOC3 Ct2. No staining reminiscent of OOC- 3 could be observed. (i) OOC-3, (j) DNA. Bar, 10 mm.

Picture from Nehrke K et al. (2002) J Biol Chem "The NHX family of Na+-H+ exchangers in Caenorhabditis elegans."

O and P, transgenic nematodes expressing nhx-8::GFP in the lateral seam cells (sc).

Picture from Rose S et al. (2007) Mol Biol Cell "Caenorhabditis elegans intersectin: a synaptic protein regulating ...."

(E and F) Epifluorescence of transgenic worms carrying the pitsn-1:ITSN1::GFP array. Worms are in ventral (E) and lateral views (F), head to the left. SC, sublateral cords; C, commissures. Bar, 10 um (all panels).

Picture from Nguyen H et al. (2018) PLoS Genet "C. elegans ZHP-4 is required at multiple distinct steps in the formation of ...."

(A) alpha-ZHP-4(green) and alpha-ZHP-3 (red) immunostaining in wild-type pachytene stage nuclei showing colocalization with the SC in early and mid-pachytene followed by co-restriction to ~6 distinct foci per nucleus in late pachytene.

Picture from Hsu TY et al. (2010) Curr Biol "Engulfment of apoptotic cells in C. elegans is mediated by integrin alpha/SRC ...."

Figure 3. SRC-1 Physically Interacts with INA-1(i) and CED-2 In Vitro and Is Colocalized with INA-1 in Phagocytes to the Pseudopods Extending around Apoptotic Cells. (A) SRC-1 interacts with INA-1(i) and CED-2 in the yeast two-hybrid assay. Pairs of constructs expressing the indicated fusion proteins were transformed into the yeast strain MaV203. The resulting transformants were streaked on SC-Trp-Leu-His or SC-Trp-Leu plates containing 25 mM 3AT. Growth on the SC-Trp-Leu-His + 25 mM 3AT plate indicates interaction of fusion proteins.(Ba-Bd) INA-1::GFP and SRC-1::mCherry are colocalized to the pseudopods extending around an apoptotic cell. INA-1::GFP (Ba), SRC-1::mCherry (Bb), merged INA-1::GFP and SRC-1::mCherry (Bc), and DIC (Bd) images of the same embryo coexpressing Pced-1ina-1::gfp and Pced-1src-1::mcherry are shown. Apoptotic cells are indicated by arrows and are magnified in the insets. Scale bar in (Ba) represents 5 um.

Picture from Nguyen H et al. (2018) PLoS Genet "C. elegans ZHP-4 is required at multiple distinct steps in the formation of ...."

Fig 2. ZHP-4 and ZHP-3 colocalize and are interdependent for their localization to the SC and crossover sites. (A) alpha-ZHP-4(green) and alpha-ZHP-3 (red) immunostaining in wild-type pachytene stage nuclei showing colocalization with the SC in early and mid-pachytene followed by co-restriction to ~6 distinct foci per nucleus in late pachytene. (B) Immunostainings of midpachytene region nuclei of the indicated genotypes with alpha-ZHP-4 (green) and alpha-ZHP-3 (red). ZHP-4 could not be detectedabove background levels in zhp-3(jf61) germlines stained with DAPI (blue) and conversely no ZHP-3 could be detected in zhp-4(vv103) mutants. Scale bars 5 um.

Picture from Salzberg Y et al. (2013) Cell "Skin-derived cues control arborization of sensory dendrites in Caenorhabditis ...."

(B) Image of an adult animal, showing expression of the mnr-1::GFP reporter in the lateral and ventral hypodermal ridges, but not in seam cells. At earlier larval stages, some expression is also seen in a limited number of neurons and the anterior pharyngeal bulb. dhr, dorsal hypodermal ridge; lhr, lateral hypodermal ridge; sc, seam cells; vhr, ventral hypodermal ridge; an asterisk indicates the vulva.

Picture from Bhalla N et al. (2008) PLoS Genet "ZHP-3 acts at crossovers to couple meiotic recombination with synaptonemal ...."

ZHP-3 localizes to sites of meiotic crossover recombination. (A-F) The site from which the asymmetric disassembly of the SC occurs is marked by a ZHP-3 focus. Wild-type nuclei in diplotene/diakinesis stained with DAPI and antibodies against HTP-3, ZHP-3 and SYP-1. An individual bivalent from one of these nuclei is shown in DF. Scale bar in D represents 2 microns.

Picture from Shakes DC et al. (2009) PLoS Genet "Spermatogenesis-specific features of the meiotic program in Caenorhabditis ...."

Figure 5. Synaptonemal complex (SC) protein disassembly in sperm and oocyte meiosis. Immunolocalization of SC proteins is shown in green and DNA in red. (A) The SC central element protein, SYP-1, departs asymmetrically from chromosomes early in the transition from diplotene to diakinesis during spermatogenesis but retained on oocyte chromosomes until late diakinesis. (B) The axial/lateral element protein HIM-3 associates to chromosomes similarly in sperm and oocyte condensation zones. (C) AIR-2 is present along each chromosomal axis in pachytene and early diplotene nuclei in oogenesis and spermatogenesis. In oogenesis, AIR-2 is not detectable in late diplotene or early diakinesis but reappears in late diakinesis on the short arms of each bivalent. During spermatogenesis, AIR-2 localizes to discrete regions on karyosome then shifts in localization to the short arms of the bivalents during diakinesis. (D) AIR-2 is absent during pachytene in syp-1(me17) mutants in both oogenesis and spermatogenesis. AIR-2 reappears during spermatogenesis, but is mislocalized in an uneven distribution around univalents. AIR-2 during oogenesis reappears during diakinesis only in syp-1(me17) mutants mated with WT males in the -1 oocyte (unmated syp-1(me17) -1 oocyte is not shown) and is also mislocalized. Scale bars represent 2 um. Panels in rows corresponding to sperm or oocyte are sized the same as in (A).