figure 2. Cells that express
cdh-3 reporter constructs during embryonic morphogenesis and postembryonic development. Two different reporter constructs were used (see Materials and Methods): (A-C) animals carrying extrachromosomal copies of the pJP#19 reporter construct; (D-H) animals carrying extrachromosomal copies of the reporter construct pJP#38. In each case the bar indicates 10 µm.(A) Right lateral view of a 1.5-fold embryo. Dorsal is to the left and anterior is to the top. The lacZ-expressing nuclei of the right seam cells are labelled. The small arrow indicates the position of the lacZ-expressing
hyp10 cell. The arrowheads indicate the positions of four lacZ-expressing nuclei whose positions are consistent with those of arcade cells. (B) A later stage embryo (2-fold) showing continued lacZ expression in the seam cells. The arrow indicates the position of the rectum. (C) A ventral view of a 1.5-fold embryo, showing the large lacZ-expressing excretory cell nucleus (arrow). (D) Dorsal view of a newly hatched L1 larva. The GFP-expressing seam cells on the left side are labelled; the numbering corresponds to those of their right homologues. Note that V5L and R have divided, but the other seam cells have not, indicating that this animal was photographed approximately 5 hours after hatching. The F and U cells (just out of the focal plane) are indicated with an arrowhead and the
hyp11 and
hyp10 cells by arrows. (E) A right lateral view of an adult hermaphrodite showing GFP expression in the arcade cells. In earlier stages it is more difficult to resolve these cells. The anterior (a) and posterior (p) arcade cell bodies are indicated. The other arcade cell bodies (three from the posterior arcade and one from the anterior arcade) are not visible in this focal plane. (F) Lateral view of the rectum of an L2 larva. The GFP-expressing F and U cells are indicated by large arrows. The small arrow shows the position of the anus. (G) Residual GFP expression in the excretory cell of a newly hatched L1 larva (right lateral view, anterior is to the right). The arrow indicates the cell body. Triangles indicate the anteriorly and posteriorly extended lateral processes; only the right lateral processes are visible in this focal plane. (H) GFP expression in the
hyp10 and
hyp11 cells of the tail of an L1 larva.