Picture from Hyun M et al. (2016) Genetics "BLIMP-1/BLMP-1 and Metastasis Associated Protein (MTA1) Regulate Stress ...."

(A and B) The levels of blmp-1 mRNA measured by qPCR (A) and BLMP-1 protein measured by Western blot analysis (B) are highest at L2 stage compared to other stages in wild-type C. elegans. In A, the values are average 6 SEM of three independent experiments. Em, embryos;YA, young adults. In B, actin is shown as a loading control.

Picture from Luo L et al. (2011) Mol Biol Cell "The Caenorhabditis elegans GARP complex contains the conserved Vps51 subunit ...."

Figure 3: GARP complex localizes to the Golgi endosomal interface. (A) GARP complex localization overlaps with the medial Golgi (Mans::GFP) and late Golgi (GFP::GRIP domain) and partially with endosomal domains (GFP::2xFYVE) in body-wall muscles. Insets show magnifications of the marked regions of the merged fluorescence images. Scale bar, 5 μm. (B) Thin plastic section EM picture of a Golgi ministack present in C. elegans body-wall muscles. Scale bar, 200 nm. (C) GARP complex localization overlaps with the medial Golgi (Mans::GFP) but not with endosomal domains (2xFYVE::GFP) in coelomocytes. Scale bar, 5 μm. (D) Thin plastic section EM picture of Golgi stacks present in C. elegans coelomocytes. Scale bar, 500 nm. (E) VPS-52::mCherry can be colocalized with the C. elegans Rab6 orthologues GFP::RAB-6.1 and GFP::RAB-6.2. (F) VPS-52 can be coimmunoprecipitated with both Rab6 orthologues; GFP::RAB-10 was used as a negative control. Scale bar, 5 μm.

Picture from Schriever AM et al. (1999) J Biol Chem "CLC chloride channels in Caenorhabditis elegans."

GFP expression directed by CeCLC promoter fragments in transgenic animals. G-I, ceclc-3:: GFP expression. G, larva with its head to the lower right; H, several adult hermaphrodites lying side by side, with the animal at bottom pointing its head to the left; I, higher magnification of a central part of a hermaphrodite. The H-shaped excretory cell (ec), enteric muscles (em), the first four epithelial cells of the intestine (ic) and the HSN neurons (HSN) are labeled. J-L, DIC photomicrographs corresponding to images G-I.

Picture from Bandyopadhyay J et al. (2002) Mol Biol Cell "Calcineurin, a calcium/calmodulin-dependent protein phosphatase, is ...."

Immunogold electron micrograph (EM) showing the subcellular localization of CNB-1. (E) Transverse section through the midbody of a hermaphrodite showing CNB-1 localization as indicated by the signals of gold particles in the seam cells of the lateral hypodermis (LH) closely apposed to the cuticle (arrowhead). (F) Another region of seam cell showing signals. Lateral hypodermis (LH), cuticle (arrowhead), and excretory canal (arrow) are labeled. (G) Localization of CNB-1 in a wild-type male gonad. Distinct and scattered signals of CNB-1 are observed mainly in the cytoplasmic regions of the cellularized spermatids. Bar, 1 um.

Picture from Desai A et al. (2003) Genes Dev "KNL-1 directs assembly of the microtubule-binding interface of the kinetochore ...."

(C) Projected 3D data sets of fixed embryos at different cell cycle stages stained for microtubules and DNA (red and cyan; left column), the kinetochore marker CeCENP-C (second column), and KNL-1 (third column). The merged images on the far right show CeCENP-C (red) and KNL-1 (green) at 2.5x higher magnification. (D) Immuno-EM of KNL-1 in multicellular embryos prepared by high-pressure freezing/freeze substitution. Insets were magnified 2x. The schematic to the right illustrates the positions of the gold particles in the field of view. Bars: C, 10 μm; D, 500 nm.

Picture from Haklai-Topper L et al. (2010) Gene Expr Patterns "The neurexin superfamily of Caenorhabditis elegans."

Fig. 3. itx-1 expression. (A): itx-1prom::gfp expression in non-neuronal cells. (a) Intestinal expression in embryo and (b) in an L1 larva. (c) expression in spermatheca (white arrows) and (d) in vulva (white arrows). Blue star in (c) and (d) indicates gut expression. (B): itx-1prom::gfp expression in glial cells. (a) Expression in amphid socket cells; gfp signal is inverted for contrast; (b-e) co-labeling of itx-1-expressing sheath cells and sensory neurons filled with dye, as previously described (Perkins et al., 1986) (b), socket cells with itx-1prom::gfp (c), Normarksi image (d) and overlap of red and green in (e); (f): Sensory anatomy with associated sheath cell nuclei labeled in blue, socket cell nuclei in green and sensory neuron nuclei in red. Adapted from (Ward et al., 1975).

Picture from Yuan JY et al. (1993) Cell "The C. elegans cell death gene ced-3 encodes a protein similar to mammalian ...."

Figure 2. ted-3 RNA Is Expressed in Embryos.A Northern blot of poly(A)+ RNA from mixed stages, embryos, Ll-L4 larvae, and young adults, probed with the ted-3 cDNA subclone pJl18 (see Experimental Procedures). pJ118 did not detectably hybridize to RNA derived from glpl(q237) adults (which lack a germline; Austin and Kimble, 1987) (data not shown), suggesting that the ted-3 RNA detected in the young adults in this experiment was derived from em- bryos within these animals. The level of RNA in each lane can be estimated based upon hybridization to a control actin 1 probe (Krause and Hirsh, 1984).

Picture from Wood JF et al. (2019) MicroPubl Biol "unc-42 regulates the expression of ASH terminal fate markers"

Figure 1: unc-42 regulates terminal gene battery expression in the ASH nociceptors: unc-42 function is required for the expression of ASH terminal differentiation gene marker expression specifically in the ASH neuron pair; expression in other neurons did not appear to be affected. The ASH neurons were identified by positional labeling with the lipophilic dye DiD, as previously described (Perkins et al. 1986). (A-G) Images of GFP reporter expression were obtained with a Zeiss Axio Imager Z1 Microscope, high resolution AxioCam MRm digital camera and Zeiss AxioVision software. Left panels indicate ASH neurons in wild-type animals, indicated by arrows. Right panels show the lack of expression in the ASH neurons of unc-42(gk598) mutant animals, indicated by dashed circles. Bar graphs show the percentage of animals examined showing GFP marker expression in 2, 1 or neither of the ASH neurons. A mixed age population of n > 40 animals was examined for each transgene.

Picture from Lints R et al. (1999) Development "Patterning of dopaminergic neurotransmitter identity among Caenorhabditis ...."

Expression of the cat-2::gfp reporter in dopaminergic cells. CAT-2::GFP expression in head neurons in wild-type animals transformed by EM#282 (dorsal view). (E) CAT-2::GFP in the tail of a wild-type adult male (ventral view). Fluorescence in the rays just short of the tips is consistent with localization in the dendritic endings of the A-type neurons, which, unlike the dendritic endings of the B-type neurons, do not extend into the opening at the ray tip (Sulston et al., 1980; Chow et al., 1995). Fluorescence in the body is from the cell bodies of R5A, R7A and R9A and the socket cells of the copulatory spicules (SpSo). Scale bar in C = 10 um.

Picture from Haklai-Topper L et al. (2010) Gene Expr Patterns "The neurexin superfamily of Caenorhabditis elegans."

Fig. 5. ITX-1 protein localization. (A): ITX-1 localizes to adherence junctions in the intestine. anti-ITX-1 immunostaining of an L1 larval stage animal is shown. (B-C): Immuno-EM with anti-ITX-1 antibodies. Arrows point to the electron dense gold particles of ITX-1 antibody that is situated in close vicinity to the typical C. elegans adherens junction and along the baso-lateral membrane of intestinal cells. (D-F): ITX-1 antibody staining in the intestine, along the apical junction appears as a wider belt than the adherens junction marked using an antibody to AJM-1. (G-M): Co-localization of ITX-1 and b-G Spectrin in the intestine (G-J) and the pharynx (K-L). Panels F, I and M are merges of the preceding red and green images.