Figure 1. Defective-pharynx and constipated mutants are resistant to virus infection:(A) Proportion of animals showing infected intestinal cells (stained by FISH against Orsay virus RNA2) 72 hours post-inoculation (hpi) with the virus. One experiment was performed with three biological replicates per condition. For each biological replicate, 100 animals were scored. Data are presented as mean ± standard error. Significance was calculated using a general linear model where the genotype was the fixed factor. Stars indicate the significance of the difference between the mutants and the N2 nematode strain reference (*** P < 0.001). (B) Venn diagram representing the overlap of differentially-expressed genes in
eat-2(
ad465) mutants and the most expressed genes of the C. elegans intracellular pathogen response (IPR), as defined by Reddy and colleagues (2019). The left panel uses
eat-2(
ad465) transcriptional data from Heestand and colleagues (2013) and the right one
eat-2(
ad465) data from Gao and colleagues (2018). Genes that are differentially expressed in both studies are highlighted in green text (C) Proportion of animals showing infected intestinal cells 72 hpi. One experiment was performed with three biological replicates per condition. For each biological replicate, 100 animals were scored. Data are presented as mean ± standard error. Significance was calculated using a general linear model where the genotype and the bacteria were the fixed factors. Tukey contrasts were used for post hoc analyses. Stars indicate the significance of the difference, in each bacterium, between the mutants and the N2 nematode strain reference (*** P < 0.001). (D) Proportion of animals showing infected intestinal cells 72 hpi. Panel in the left and panel in the right represent independent experiments. Each experiment was performed with three biological replicates per condition. For each biological replicate, 100 animals were scored. Data are presented as mean ± standard error. Significance was calculated using a general linear model where the genotype was the fixed factor. Tukey contrasts were used for post hoc analyses. Stars indicate the significance of the difference, in each bacterium, between the mutants and the N2 nematode strain reference (*** P < 0.001). (E) Nomarski micrographs of the intestine of young adults (3 days after embryo) of different genotypes cultured in E. coli OP50. Yellow arrows point to bacteria present in the gut. Scale bar represents 10 microns.