Figure 5.
png-1 is widely expressed. A-F,
png-1 promoter activity at various developmental stages visualized using a Ppng-1::GFP transcriptional reporter (see Fig. 2A). The ~600 bp promoter region was shown to be sufficient to rescue VC branching defects in
png-1 mutants. Promoter activity was found in multiple tissues, including epithelium, neurons, muscle, intestine, and vulval epithelial cells. Inset in B shows magnification of L3 vulval region. G, H, PNG-1::GFP expressed from a pan-epithelial or pan-neuronal promoter shows cytoplasmic localization. Epithelial seam cells (G) and a tail neuron (H) are shown. Scale bars, 10 um.