Picture from Osborne, Jennifer F et al. (2021) MicroPubl Biol

Figure 1. Assessment of genetic interactions: (A) ALS genes of interest (left) and corresponding deletion alleles in C.elegans orthologs (right). (B) Representative images from sod-1WTc (left) and sod-1G85Rc (right) after 22 hours of paraquat exposure. Animals with three or four phasmid neurons that fill with dye are scored as having no degeneration. Animals are outlined, fluorescence in the gut is visible in each image, and location of missing neurons is outlined in sod-1G85Rc . 63x magnification, scale bar 20um. (C) Glutamatergic neuron degeneration: after 22 hours of paraquat exposure, an increased percentage of figo-1(tm5202); sod-1G85Rc double mutant animals showed no degeneration, compared to sod-1G85Rc animals. Three independent trials totalling n=30 animals, two-tailed t-test, p=0.03. Dots represent the average from each trial of 10 animals. Error bars indicate standard error of the mean. All data were collected blind to genotype.(D-G) Glutamatergic neuron degeneration as described in C for other genotypes.

Picture from Lee J et al. (2004) J Mol Biol "Opposing functions of calcineurin and CaMKII regulate G-protein signaling in ...."

Calcineurin A expression in hypodermal cells was observed by (A) GFP expression as well as (B) immunostaining.

Picture from Pauli F et al. (2006) Development "Chromosomal clustering and GATA transcriptional regulation of ...."

Intestinal expression of D2030.5 (mce-1). Expression is shown in the intestine as well as other tissues.

Picture from Sze JY et al. (2003) Proc Natl Acad Sci U S A "Activity of the Caenorhabditis elegans UNC-86 POU transcription factor ...."

In wild-type animals, the lin-11::GFP is expressed in the AIZ interneurons as well as several other head neurons.

Picture from Nelson MD et al. (2011) PLoS Genet "A bow-tie genetic architecture for morphogenesis suggested by a genome-wide ...."

RME-8::GFP localizes to the edge of detaching and retracting tail tip cells as puncta (depicted as dark green dots in the schematic).

Picture from Meissner B et al. (2011) PLoS One "Determining the sub-cellular localization of proteins within Caenorhabditis ...."

Sub-cellular localization pattern for F42C5.9. F42C5.9 is present in the cell membrane, as well as filament-like structures and dense body-like structures. Bar 10 um.

Picture from Stitzel ML et al. (2007) Curr Biol "Regulation of MBK-2/Dyrk kinase by dynamic cortical anchoring during the ...."

(C) Images of live embryos expressing GFP fusions as indicated.

Picture from Nath RD et al. (2016) Curr Biol "C.elegans Stress-Induced Sleep Emerges from the Collective Action of Multiple ...."

(E) DIC image of the ALA and RID neurons (white arrows). As previously reported, ALA is posterior to RID [S2]. (F) Overlay of nlp-8 gfp reporter expression on DIC image. nlp-8 is expressed in ALA, as well as other neurons in the head [S3].

Picture from Schonegg S et al. (2007) Proc Natl Acad Sci U S A "The Rho GTPase-activating proteins RGA-3 and RGA-4 are required to set the ...."

(C) A wild-type embryo and a rga-3/4(RNAi) embryo stained with anti-RGA-3 antibody. RGA-3 localizes to the cortex as well as to centrosomes.

Picture from Ooi SL et al. (2006) PLoS Genet "Histone H3.3 variant dynamics in the germline of Caenorhabditis ...."

HIS-71::GFP fluorescence in living adults; cell types as indicated.