Picture from Oomura, Shun et al. (2022) MicroPubl Biol

Figure 1. Microparticle bombardment with hygromycin B screening for C. inopinataA. Plasmids for microparticle bombardment. They contain transgenes and the hygromycin resistance cassette. B. Time schedule for microparticle bombardment and hygromycin B screening for C. inopinata. After the bombardment of young adults of C. inopinata, worms were distributed to 10 recovery plates per one shot. On Day 2, hygromycin B was added to each plate. Within three weeks, most non-transformed worms and transient transformants harboring extrachromosomal arrays were killed by hygromycin B, and survivors could be isolated as candidates for transgene integrants. C-E. Fluorescent images of C. elegans and C. inopinata integrant strains expressing GFP markers. C. GFP::H2B histone in hermaphrodite/female adults. Scale bars = 200 μm. D. GFP::PH domain in adult hermaphrodite/female gonads. Scale bars = 40 μm. E. GFP::TBB-2 in one- cell embryos. Scale bars = 10 μm.