Picture from Olson AC et al. (2019) MicroPubl Biol

Figure 1: Time course of paralysis induced by treatment with serotonin in wild-type and mutant strains of C. elegans. Animals were placed in M9 liquid medium containing 10 mM serotonin and the animals still moving were counted at the indicated times. 100 animals were tested for each genotype. Error bars, 95% confidence intervals. Panel A and B assays were carried out on separate days. Some genotypes are shown in both panels to demonstrate the reproducibility of the assay. The elpc-3(ok2452) and mod-5(n3314) mutants are control strains previously shown (Grel et al. 2012) to be serotonin resistant (elpc-3) or serotonin hypersensitive (mod-5). A) Loss-of-function mutants of egl-4(n479), pkg-2(tm3878), and pkg-2(tm5814) are resistant to serotonin-induced paralysis; B) the egl-4(mg410) gain-of-function (gf) mutant is hypersensitive to serotonin-induced paralysis.

Picture from Olson SK et al. (2012) J Cell Biol "Hierarchical assembly of the eggshell and permeability barrier in C. ...."

(C) CPG-1 and CPG-2 are secreted CPGs composed of a protein core (blue) with covalently linked chondroitin side chains (thin black lines are putative chondroitin attachment sites based on sequence consensus; Olson et al., 2006) and chitin-binding domains. Images are electron micrographs of eggshells after immunogold labeling (10-nm beads) using antibodies recognizing both CPG-1 and CPG-2 (top) or specifically CPG-2 (bottom). Bars, 100 nm.

Picture from Olson SK et al. (2012) J Cell Biol "Hierarchical assembly of the eggshell and permeability barrier in C. ...."

Figure 1. Chitin and the CPG-1/2 localize to the middle and inner layers of the trilaminar eggshell, respectively. (middle) Transmission electron micrographs of the eggshell in high-pressure frozen embryos. (right) Pseudocolored micrographs illustrate the location of the outer (black), middle, and inner eggshell layers. Beneath the inner layer is the perivitelline space between the eggshell and embryo plasma membrane. (A, left) A schematic is provided for orientation, with the black box highlighting the imaged region. (B) Chitin is composed of repeating units of beta 1,4-linked N-acetylglucosamine. Image is an electron micrograph of an eggshell after immunogold labeling (10-nm gold beads) with a chitin-binding probe. (C) CPG-1 and CPG-2 are secreted CPGs composed of a protein core (blue) with covalently linked chondroitin side chains (thin black lines are putative chondroitin attachment sites based on sequence consensus; Olson et al., 2006) and chitin-binding domains. Images are electron micrographs of eggshells after immunogold labeling (10-nm beads) using antibodies recognizing both CPG-1 and CPG-2 (top) or specifically CPG-2 (bottom). Bars, 100 nm.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.

Picture from Millonigg S et al. (2014) PLoS Genet "GLD-4-mediated translational activation regulates the size of the ...."

(A) GLD-4 expression is equal across the distal germ line. GLD-2 intensities increase from low-to-high in a distal-to-proximal manner. Extruded gonads of indicated genotype stained with DAPI, a-GLD-2, a-GLD-4, and a-GLH-2 as a positive tissue penetration control (not shown). Asterisk, distal tip; arrowhead, mitosis-to-meiosis boundary.

Picture from Xu XZS et al. (2003) Cell "A C. elegans sperm TRP protein required for sperm-egg interactions during ...."

Figure 3. The TRP-3 Protein Is Highly Enriched in Sperm. (A-H) An affinity-purified antibody against TRP-3 specifically stains sperm in wild-type hermaphrodites. Scale bar = 10 μm.(A) An immunofluorescence image of anti-TRP3 staining of a wild-type hermaphrodite.(B) The same animal in (A) stained with DAPI.(C) A superimposed image of (A) and (B).(D) A DIC image of the same animal in (A-C).(E-H) The TRP-3 antibody is specific.(E) The TRP-3 antibody does not detect a signal in trp-3(sy693) mutants.(F) A DAPI image of the same animal shown in (E).(G) A superimposed image of (E) and (F).(H) A DIC image of the same animal shown in (E-G).