Picture from Ohkura K et al. (2003) Development "SDF-9, a protein tyrosine phosphatase-like molecule, regulates the L3/dauer ...."

Expression pattern of sdf-9. (A) Expression of sdf-9p::GFP2 (pKOG9) in the head region of an L3 larva. The left-hand cell, which is in focus in this micrograph, is strongly fluorescent, while the right-hand cell, which is also strongly fluorescent, is out of focus. The arrows indicate short dendrite-like structures. (B) Expression of sdf-9p::GFP2 expression in the head region of an L1 larva. The right-hand cell that express the GFP fusion gene is indicated. The arrow shows the dendrite-like process, which extends to the tip of the head. The images in the right white boxes in (A) and (B) are magnified versions of those in the left white boxes, respectively. Scale bar: 10 um.

Picture from Ohkura K et al. (2003) Development "SDF-9, a protein tyrosine phosphatase-like molecule, regulates the L3/dauer ...."

Figure 5. Expression pattern of sdf-9. (A) Expression of sdf-9p::GFP2 (pKOG9) in the head region of an L3 larva. The left-hand cell, which is in focus in this micrograph, is strongly fluorescent, while the right-hand cell, which is also strongly fluorescent, is out of focus. The arrows indicate short dendrite-like structures. (B) Expression of sdf-9p::GFP2 expression in the head region of an L1 larva. The right-hand cell that express the GFP fusion gene is indicated. The arrow shows the dendrite-like process, which extends to the tip of the head. (C) Expression of SDF-9::GFP (pKOG7) in two head cells of a daf-9(e1406) dauer larva. One of the two dendrite-like processes extends posteriorly. Both cells have processes, but those of the left-hand cell are out of focus. The images in the right white boxes in (A) and (B) are magnified versions of those in the left white boxes, respectively, and the bottom image is a magnified version of that in the white box in (C). Scale bar: 10 um.

Picture from Ohkura K et al. (2011) Biochem Biophys Res Commun "Dye-filling of the amphid sheath glia: implications for the functional ...."

Fig. 2. F52E1.2SP::YFP is secreted into the amphid sheath channel and/or is in the amphid sheath cell near the amphid sheath lumen. Wild-type adult animal has two extrachromosomal arrays, Ex[daf-6p::mCherry, gpc-1p::CFP, pRF4, pUC19] and Ex[F52E1.2SP::YFP, sdf-9p::dsRed, pUC19]. (A) daf-6p::mCherry is expressed in the amphid socket and sheath cells, and cytoplasmically localized. (B) gpc-1p::CFP is expressed in neurons including ASJ, ADL and ASH sensory neurons, and shows cytoplasmic localization. Dendrite ending of the ASH neuron is out of focus. (C) Some F52E1.2SP::YFP protein is transported to the amphid sheath cell ending. (D) DIC image. (E) A and B merged. Cilia are located in the amphid sheath channel. (F) B and C merged. Some F52E1.2SP::YFP protein is localized around the cilia. (G) A and C. Some F52E1.2SP::YFP protein seems to be secreted into the amphid sheath channel. (H) Schematic diagram of the amphid sensory ending.

Picture from Ohkura K et al. (2011) Biochem Biophys Res Commun "Dye-filling of the amphid sheath glia: implications for the functional ...."

Fig. 1. DiI-staining of the AMsh cells. Anterior is right (A-D) or left (E-G, I, J). The concentration of DiI is 0.01 lg/ml. (A) Granular structures shown are DiI-staining in the AMsh cells. Arrowheads show DiI-filling in specific amphid sensory neurons. Asterisk indicates sdf-9p::DsRed expression in the XXX cell, although it is out of focus. (B) F52E1.2SP::YFP expression in the amphid sheath cell is shown and dotted YFP signals are seen. (C) A and B merged. Although granules of DiI-staining in the amphid are not completely superimposed on the F52E1.2SP::YFP dots, some DiI signals are overlapping with YFP fluorescence (the arrow shows an example). Asterisk: sdf-9p::DsRed. (D) DIC image of A and B. (E) Granular structures of DiI-staining along the dendrites of the amphid sensory neurons filled with DiI. Bracket shows DiI-staining granules. Arrowheads indicate dendrites of sensory neurons stained with DiI. Because the cell body of the amphid sheath cell is out of focus, no DiI granules are seen in this image, but the cell body also contains DiI granular structures. (F) F52E1.2SP::YFP-expressing amphid sheath cell. Bracket shows YFP granules in the process. Because the cell body is not in focus, no granules are seen in this image, but the YFP granular structures also exist in the cell body. (G) Merged image shows that granular structures of DiI-staining are located in the AMsh process and that some granules are overlapping with F52E1.2SP::YFP (arrows). (H) Scheme of F52E1.2, which encodes a C-type lectin domain-containing protein expressed in the AMsh and PHsh cells. F52E1.2SP::YFP is a reporter construct used to visualize the region around the amphid sheath channel. SP: signal peptide. (I and J) Strong DiI-staining of the AMsh cells in a che-14 mutant adult. DiI: 5 ug/ml. AMsh cells in che-14 mutants are sometimes very strongly stained with DiI and the appearance is different from the granular staining shown in (A) although granular structures are also seen in the process and the cell body. Further, this staining does not correlate with the DiI-staining of amphid sensory neurons.

Picture from Solari F et al. (1999) Development "The Caenorhabditis elegans genes egl-27 and egr-1 are similar to MTA1, a member of ...."

(J,K) egl-27::gfp expression in 1.5-fold embryo (J) and L3 hermaphrodite (K). All somatic cells appear to express the reporter gene.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(K) Pgrl-8::GFP is expressed in two OLL neurons.

Picture from Huang CY et al. (2012) PLoS One "C. elegans EIF-3.K promotes programmed cell death through CED-3 ...."

Figure 2. EIF-3.K protein expression. (A) Western blot analysis of EIF-3.K protein expression. Affinity-purified anti-EIF-3.K antibodies were used to probe a blot of embryonic extracts from wild-type and eif-3.K(gk126) worms (above). Equal loading of the two extracts was confirmed by anti-a tubulin antibodies (below). The sizes of molecular weight markers and the positions of EIF-3.K and a tubulin are indicated. (B-D) Images of an eif-3.K(gk126)mutant early embryo (B), a wild-type early embryo (C) and a wild-type newly hatched larva (D) that were co-stained with anti-EIF-3.K antibodies and DAPI. Merged images are also shown. Scale bar = 10 um.

Picture from Manil-Segalen M et al. (2014) Dev Cell "The C. elegans LC3 acts downstream of GABARAP to degrade autophagosomes by ...."

(D-K) Electron micrographs of GFP::LGG-1 (D-H) or GFP::LGG-2 (J and K) embryos incubated with anti-LGG-1 (D and E) or anti-GFP (F-H, J, and K) antibodies revealing autophagosomes (D, F, and J), isolation membranes (E, G, and K) andoccasionally, the lumen of lysosomes (H). (D0-K0) Higher magnifications of the positive structures observed in (D-K). Scale bar represents 200 μm. (I) Quantification of the diameter of the autophagosomes and of the localization of gold beads are shown as mean ± SEM.

Picture from Li P et al. (2016) Neuron "Two Clathrin Adaptor Protein Complexes Instruct Axon-Dendrite ...."

(J-K) GFP-tagged SER-1 is co-expressed with a cytosolic tdTomato in AVE in wild type (J), apb-3(ok429)(K) animals. Arrows point to the dendritic region of the AVE neuron. Scale bar is 10um.

Picture from Gravato-Nobre MJ et al. (2016) PLoS Pathog "The Invertebrate Lysozyme Effector ILYS-3 Is Systemically Activated in ...."

(J-K) Representative images of the 4.5 Kb ilys-3 long promoter GFP construct in adult hermaphrodites. (J) Promoter activity in pharynx and intestine and (K) in the coelomocytes. ph:pharynx; int: intestine; cc: coelomocytes. Scale bars as indicated.